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Rachel E Rigby, Lauren M Webb, Karen J Mackenzie, Yue Li, Andrea Leitch, Martin AM Reijns, Rachel J Lundie, Ailsa Revuelta, Donald J Davidson, Sandra Diebold, Yorgo Modis, Andrew S MacDonald, Andrew P Jackson
Sep 04, 2020
The sensing of nucleic acids by receptors of the innate immune system is a key component of antimicrobial immunity. RNA:DNA hybrids, as essential intracellular replication intermediates generated during infection, could...
Animals Blotting Western Dendritic Cells Endosomes Enzyme-Linked Immunosorbent Assay Flow Cytometry Fluorescence Polarization Fluorescent Antibody Technique Humans Immunity Innate Immunoblotting Mice Mice Inbred C57BL Models Immunological Myeloid Differentiation Factor 88 Nucleic Acid Heteroduplexes Real-Time Polymerase Chain Reaction Signal Transduction Toll-Like Receptor 9
A new role for lipocalin prostaglandin d synthase in the regulation of brown adipose tissue substrate utilization.
Sam Virtue, Helena Feldmann, Mark Christian, Chong Yew Tan, Mojgan Masoodi, Martin Dale, Chris Lelliott, Keith Burling, Mark Campbell, Naomi Eguchi, Peter Voshol, Jaswinder K Sethi, Malcolm Parker, Yoshihiro Urade, Julian L Griffin, Barbara Cannon, Antonio Vidal-Puig
Feb 18, 2021
In this study, we define a new role for lipocalin prostaglandin D synthase (L-PGDS) in the control of metabolic fuel utilization by brown adipose tissue (BAT). We demonstrate that L-PGDS expression in BAT is positively...
Producing molecular biology reagents without purification.
Sanchita Bhadra, Vylan Nguyen, Jose-Angel Torres, Shaunak Kar, Stéphane Fadanka, Chiara Gandini, Harry Akligoh, Inyup Paik, Andre C Maranhao, Jenny Molloy, Andrew D Ellington
Jul 02, 2021
We recently developed 'cellular' reagents-lyophilized bacteria overexpressing proteins of interest-that can replace commercial pure enzymes in typical diagnostic and molecular biology reactions. To make cellular reagent...
covid-19 COVID-19 Testing Cameroon Diagnostic Tests Routine Escherichia coli Gene Expression Geobacillus stearothermophilus Ghana Humans Indicators and Reagents Molecular Diagnostic Techniques Plasmids Real-Time Polymerase Chain Reaction Recombinant Proteins SARS-CoV-2 synthetic biology Transformation Bacterial United Kingdom
Fibroblast growth factor receptor 1 is principally responsible for fibroblast growth factor 2-induced catabolic activities in human articular chondrocytes.
Dongyao Yan, Di Chen, Simon M Cool, Andre J van Wijnen, Katalin Mikecz, Gillian Murphy, Hee-Jeong Im
Oct 12, 2011
Blotting Western Cartilage Articular Cell Proliferation Cell Separation Cells Cultured Chondrocytes Extracellular Signal-Regulated MAP Kinases Fibroblast Growth Factor 2 Flow Cytometry Humans Immunoprecipitation Osteoarthritis RNA Small Interfering Real-Time Polymerase Chain Reaction Receptor Fibroblast Growth Factor Type 1 Receptor Fibroblast Growth Factor Type 3 Signal Transduction Transfection
HPV16 and 18 genome amplification show different E4-dependence, with 16E4 enhancing E1 nuclear accumulation and replicative efficiency via its cell cycle arrest and kinase activation functions
Cell Cycle Checkpoints Cells Cultured Enzyme Activation Fluorescent Antibody Technique Gene Amplification Genome Viral Human papillomavirus 16 Human papillomavirus 18 Humans Immunoblotting Immunohistochemistry In Situ Hybridization Fluorescence Keratinocytes Life Cycle Stages Mutagenesis Site-Directed Oncogene Proteins Viral Real-Time Polymerase Chain Reaction Virus Replication
Detection of malaria sporozoites expelled during mosquito sugar feeding.
VA Brugman, M Kristan, MP Gibbins, F Angrisano, KA Sala, JT Dessens, AM Blagborough, T Walker
Jan 31, 2020
Malaria is a severe disease of global importance transmitted by mosquitoes of the genus Anopheles. The ability to rapidly detect the presence of infectious mosquitoes able to transmit malaria is of vital importance for...
Hepatic microRNA Expression by PGC-1α and PGC-1β in the Mouse.
Elena Piccinin, Maria Arconzo, Giusi Graziano, Michele Vacca, Claudia Peres, Elena Bellafante, Gaetano Villani, Antonio Moschetta
Feb 03, 2020
The fine-tuning of liver metabolism is essential to maintain the whole-body homeostasis and to prevent the onset of diseases. The peroxisome proliferator-activated receptor-γ coactivators (PGC-1s) are transcriptional key players...
Suppression of Aβ toxicity by puromycin-sensitive aminopeptidase is independent of its proteolytic activity.
Antonina J Kruppa, Stanislav Ott, Dhia S Chandraratna, James A Irving, Richard M Page, Elena Speretta, Tiffany Seto, Luiz Miguel Camargo, Stefan J Marciniak, David A Lomas, Damian C Crowther
May 18, 2018
The accumulation of β-amyloid (Aβ) peptide in the brain is one of the pathological hallmarks of Alzheimer's disease and is thought to be of primary aetiological significance. In an unbiased genetic screen, we identified...
Alzheimer Amyloid Autophagy Proteolysis Puromycin-sensitive aminopeptidase Alzheimer Disease Aminopeptidases Amyloid beta-Peptides Animals Animals Genetically Modified Autophagy Blotting Western Brain Drosophila melanogaster Enzyme-Linked Immunosorbent Assay Flow Cytometry Fluorescent Antibody Technique Humans Immunoenzyme Techniques Neuroblastoma Neurons Proteolysis puromycin RNA Messenger Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Tumor Cells Cultured
AKR1C3-mediated adipose androgen generation drives lipotoxicity in women with polycystic ovary syndrome
MW O'Reilly, P Kempegowda, M Walsh, AE Taylor, K Manolopoulos, JW Allwood, RK Semple, D Hebenstreit, WB Dunn, JW Tomlinson, W Arlt
Aug 29, 2017
3-Hydroxysteroid Dehydrogenases Adipocytes Adolescent Adult Aldo-Keto Reductase Family 1 Member C3 Analysis of Variance Androgens Area Under Curve Biomarkers Case-Control Studies Cells Cultured Dehydroepiandrosterone Female Humans Hydroxyprostaglandin Dehydrogenases Insulin Resistance Lipid Metabolism Lipolysis POLYCYSTIC OVARY SYNDROME Real-Time Polymerase Chain Reaction Reference Values statistics Nonparametric Subcutaneous Fat Young Adult
Are brucellosis, Q fever and melioidosis potential causes of febrile illness in Madagascar?
Ides Boone, Klaus Henning, Angela Hilbert, Heinrich Neubauer, Vera von Kalckreuth, Denise Myriam Dekker, Norbert Georg Schwarz, Gi Deok Pak, Andreas Krüger, Ralf Matthias Hagen, Hagen Frickmann, Jean Noël Heriniaina, Raphael Rakotozandrindrainy, Jean Philibert Rakotondrainiarivelo, Tsiry Razafindrabe, Benedikt Hogan, Jürgen May, Florian Marks, Sven Poppert, Sascha Al Dahouk
Feb 19, 2019
Brucellosis, Q fever and melioidosis are zoonoses, which can lead to pyrexia. These diseases are often under-ascertained and underreported because of their unspecific clinical signs and symptoms, insufficient awareness by...
Robust derivation of epicardium and its differentiated smooth muscle cell progeny from human pluripotent stem cells.
Dharini Iyer, Laure Gambardella, William G Bernard, Felipe Serrano, Victoria L Mascetti, Roger A Pedersen, Amarnath Talasila, Sanjay Sinha
May 18, 2015
DACH1, a zona glomerulosa selective gene in the human adrenal, activates transforming growth factor-β signaling and suppresses aldosterone secretion.
Junhua Zhou, Lalarukh Haris Shaikh, Sudeshna G Neogi, Ian McFarlane, Wanfeng Zhao, Nichola Figg, Cheryl A Brighton, Carmela Maniero, Ada ED Teo, Elena AB Azizan, Morris J Brown
May 19, 2015
DACH1 protein Human aldosterone hyperaldosteronism Transforming Growth Factor beta zona glomerulosa Adrenocortical Carcinoma Aldosterone Disease Progression Eye Proteins Female Gene Expression Regulation Neoplastic Humans Immunohistochemistry Male Middle Aged RNA Neoplasm Real-Time Polymerase Chain Reaction Signal Transduction Transcription Factors Transforming Growth Factor beta Tumor Cells Cultured Zona Glomerulosa
BET protein inhibition shows efficacy against JAK2V617F-driven neoplasms.
BS Wyspiańska, AJ Bannister, I Barbieri, J Nangalia, A Godfrey, FJ Calero-Nieto, S Robson, I Rioja, J Li, M Wiese, E Cannizzaro, MA Dawson, B Huntly, RK Prinjha, AR Green, B Gottgens, T Kouzarides
Jun 13, 2014
Regulation of the clock gene expression in human adipose tissue by weight loss.
O Pivovarova, Ö Gögebakan, S Sucher, J Groth, V Murahovschi, K Kessler, M Osterhoff, N Rudovich, A Kramer, AFH Pfeiffer
Aug 05, 2019
BACKGROUND: The circadian clock coordinates numerous metabolic processes to adapt physiological responses to light-dark and feeding regimens and is itself regulated by metabolic cues. The implication of the circadian clock in...
ARNTL Transcription Factors Adipose Tissue Adult CLOCK Proteins Caloric Restriction Circadian Clocks Female Gene Expression Regulation Humans Lipid Metabolism Male Nuclear Receptor Subfamily 1 Group D Member 1 obesity Period Circadian Proteins Real-Time Polymerase Chain Reaction Subcutaneous Fat Abdominal Weight Loss
Tissue transglutaminase mediates the pro-malignant effects of oncostatin M receptor over-expression in cervical squamous cell carcinoma.
Maria M Caffarel, Anasuya Chattopadhyay, Angela M Araujo, Julien Bauer, Cinzia G Scarpini, Nicholas Coleman
Aug 19, 2020
Oncostatin M receptor (OSMR) is commonly over-expressed in advanced cervical squamous cell carcinoma (SCC), producing a significantly worse clinical outcome. Cervical SCC cells that over-express OSMR show enhanced responsiveness...
cell migration Fibronectin integrin-α5β1 invasion oncostatin M receptor squamous cell carcinoma tissue transglutaminase Blotting Western Carcinoma Squamous Cell Female Fibronectins Fluorescent Antibody Technique GTP-Binding Proteins Humans Immunohistochemistry Immunoprecipitation Integrin alpha5beta1 Microscopy Confocal Neoplasm Invasiveness Oligonucleotide Array Sequence Analysis Protein Glutamine gamma Glutamyltransferase 2 Real-Time Polymerase Chain Reaction Receptors Oncostatin M Signal Transduction Tissue Array Analysis Transglutaminases Up-Regulation Uterine Cervical Neoplasms
The SARS-CoV-2 viral load in COVID-19 patients is lower on face mask filters than on nasopharyngeal swabs.
Agnieszka Smolinska, David S Jessop, Kirk L Pappan, Alexandra de Saedeleer, Amerjit Kang, Alexandra L Martin, Max Allsworth, Charlotte Tyson, Martine P Bos, Matt Clancy, Mike Morel, Tony Cooke, Tom Dymond, Claire Harris, Jacqui Galloway, Paul Bresser, Nynke Dijkstra, Viresh Jagesar, Paul HM Savelkoul, Erik VH Beuken, Wesley HV Nix, Renaud Louis, Muriel Delvaux, Doriane Calmes, Benoit Ernst, Simona Pollini, Anna Peired, Julien Guiot, Sara Tomassetti, Andries E Budding, Frank McCaughan, Stefan J Marciniak, Marc P van der Schee
Sep 01, 2021
Face masks and personal respirators are used to curb the transmission of SARS-CoV-2 in respiratory droplets; filters embedded in some personal protective equipment could be used as a non-invasive sample source for applications...
High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology.
Laetitia Chauve, Jérémie Le Pen, Francesca Hodge, Pia Todtenhaupt, Laura Biggins, Eric A Miska, Simon Andrews, Olivia Casanueva
Jun 22, 2020
This paper presents a high-throughput reverse transcription quantitative PCR (RT-qPCR) assay for Caenorhabditis elegans that is fast, robust, and highly sensitive. This protocol obtains precise measurements of gene expression...
Transcript analysis reveals a specific HOX signature associated with positional identity of human endothelial cells.
Mark Toshner, Benjamin J Dunmore, Eoin F McKinney, Mark Southwood, Paola Caruso, Paul D Upton, John P Waters, Mark L Ormiston, Jeremy N Skepper, Gerard Nash, Amer A Rana, Nicholas W Morrell
Jun 14, 2018
The endothelial cell has a remarkable ability for sub-specialisation, adapted to the needs of a variety of vascular beds. The role of developmental programming versus the tissue contextual environment for this specialization is...
Adult Animals Cell Differentiation Cell Line Cluster Analysis Embryonic Stem Cells Endothelial Cells Fetus Gene Expression Profiling Gene Ontology Genes Homeobox Homeodomain Proteins Humans Lung Mice Neovascularization Physiologic Oligonucleotide Array Sequence Analysis Organ Specificity Phenotype Pulmonary Artery RNA Messenger Rats Real-Time Polymerase Chain Reaction Reproducibility of Results
The effects of aging and maternal protein restriction during lactation on thymic involution and peripheral immunosenescence in adult mice.
Gerotarget developmental programming immunosenescence lifespan maternal diet thymic involution aging Animal Nutritional Physiological Phenomena Animals Blotting Western Cells Cultured Diet Protein-Restricted Female Flow Cytometry Fluorescent Antibody Technique Immunosenescence Lactation Mice Mice Inbred C57BL RNA Messenger Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes Thymus Gland
Effective control of SARS-CoV-2 transmission between healthcare workers during a period of diminished community prevalence of COVID-19.
Nick K Jones, Lucy Rivett, Dominic Sparkes, Sally Forrest, Sushmita Sridhar, Jamie Young, Joana Pereira-Dias, Claire Cormie, Harmeet Gill, Nicola Reynolds, Michelle Wantoch, Matthew Routledge, Ben Warne, Jack Levy, William David Córdova Jiménez, Fathima Nisha Begum Samad, Chris McNicholas, Mark Ferris, Jane Gray, Michael Gill, CITIID-NIHR COVID-19 BioResource Collaboration, Martin D Curran, Stewart Fuller, Afzal Chaudhry, Ashley Shaw, John R Bradley, Gregory J Hannon, Ian G Goodfellow, Gordon Dougan, Kenneth GC Smith, Paul J Lehner, Giles Wright, Nicholas J Matheson, Stephen Baker, Michael P Weekes
Jun 24, 2020
Previously, we showed that 3% (31/1032)of asymptomatic healthcare workers (HCWs) from a large teaching hospital in Cambridge, UK, tested positive for SARS-CoV-2 in April 2020. About 15% (26/169) HCWs with symptoms of coronavirus...
covid-19 SARS-CoV-2 emerging pathogens EPIDEMIOLOGY Global Health human human biology infectious disease Medicine Occupational health Virology Adult Asymptomatic Diseases Betacoronavirus covid-19 COVID-19 Testing Clinical Laboratory Techniques Community-Acquired Infections Contact Tracing Coronavirus Infections Disease Transmission Infectious England Family Characteristics Female Health Personnel Hospital Units Hospitals Teaching Hospitals University Humans infection control Infectious Disease Transmission Patient-to-Professional Male Mass Screening Middle Aged nasopharynx Occupational Diseases Pandemics Patient Admission Pneumonia Viral prevalence Program Evaluation Real-Time Polymerase Chain Reaction SARS-CoV-2 Symptom Assessment
Fully automated real-time PCR for EGFR testing in non-small cell lung carcinoma.
Molecular testing for mutations in the EGFR gene is commonplace for patients with non-small cell lung cancer (NSCLC). These patients are often very sick and management decisions need to be made urgently. In many cases, the...
MacroH2A1 isoforms are associated with epigenetic markers for activation of lipogenic genes in fat-induced steatosis.
Christine Podrini, Apostolos Koffas, Shilpa Chokshi, Manlio Vinciguerra, Christopher J Lelliott, Jacqueline K White, Hibret A Adissu, Roger Williams, Azzura Greco
Apr 07, 2021
The importance of epigenetic changes in the development of hepatic steatosis is largely unknown. The histone variant macroH2A1 under alternative splicing gives rise to macroH2A1.1 and macroH2A1.2. In this study, we show that the...
bivalent promoter histone methylation histone variants Lipid uptake Animals Biomarkers Blotting Western Carcinoma Hepatocellular Cells Cultured Chromatin Immunoprecipitation Diet High-Fat Epigenomics Fatty Liver Female Fluorescent Antibody Technique Gene Expression Profiling Hepatocytes Histones Humans Immunoenzyme Techniques Lipid Peroxidation Liver Liver Neoplasms Male Mice Mice Inbred C57BL Mice Knockout Mitochondrial Proton-Translocating ATPases Oligonucleotide Array Sequence Analysis Promoter Regions Genetic Protein Isoforms RNA Messenger Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction
PERK Activation Promotes Medulloblastoma Tumorigenesis by Attenuating Premalignant Granule Cell Precursor Apoptosis.
Yeung Ho, Xiting Li, Stephanie Jamison, Heather P Harding, Peter J McKinnon, David Ron, Wensheng Lin
Mar 09, 2016
Adult Animals Apoptosis Blotting Western Carcinogenesis Cell Transformation Neoplastic Cerebellar Neoplasms Child Child Preschool Disease Models Animal Enzyme Activation Female Humans Immunohistochemistry In Situ Nick-End Labeling Infant Male Medulloblastoma Mice Mice 129 Strain Mice Inbred C57BL Mice Mutant Strains Neural Stem Cells Neurons Real-Time Polymerase Chain Reaction eIF-2 Kinase
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