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Whole-genome, deep pyrosequencing analysis of a duck influenza A virus evolution in swine cells.
Vincent Bourret, Guillaume Croville, Jérôme Mariette, Christophe Klopp, Olivier Bouchez, Laurence Tiley, Jean-Luc Guérin
Jun 10, 2013
EMEM Eagle’s Minimum Essential Medium FCS Influenza A virus LB Luria–Bertani MID Mixed infection NPTr Newborn Pig Trachea Pyrosequencing Reassortment SD SNP Viral evolution Within-host diversity a.f.t. allele frequency threshold base pairs bp dNTP deoxyribonucleotides triphosphate fœtal calf serum multiplex identifier sequence single nucleotide polymorphism standard deviation Animals Animals Wild Cell Line Coinfection Ducks evolution Molecular High-Throughput Nucleotide Sequencing Influenza A virus Influenza in Birds Polymorphism Single Nucleotide RNA Viral Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis DNA Swine