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Pentameric ligand-gated ion channels (pLGICs) or Cys-loop receptors are involved in fast synaptic signaling in the nervous system. Allosteric modulators bind to sites that are remote from the neurotransmitter binding site, but...

allosteric modulation human Ligand-Gated Ion Channels molecular biophysics neuroscience structural biology Bacterial Proteins Binding Sites Erwinia Ligand-Gated Ion Channels Models Molecular Mutagenesis Site-Directed Protein Conformation Receptors Serotonin 5-HT3 Single-Domain Antibodies

Influenza H7N9 virus continues to cause infections in humans and represents a significant pandemic risk. During the most recent 5th epidemic wave in 2016/17 two distinct lineages with increased human infections and wider...

Amino Acid Sequence Animals Antibodies Neutralizing Antibodies Viral Binding Sites Birds Epitopes Hemagglutinin Glycoproteins Influenza Virus Humans Influenza A virus H7N9 Subtype Influenza in Birds Influenza Human Models Molecular Peptide Library Protein Binding Protein Conformation Protein Interaction Domains and Motifs Saccharomyces cerevisiae Single-Domain Antibodies

Acrylates Analytic Sample Preparation Methods Chromatography Liquid Cross-Linking Reagents Cysteine Humans Immunoconjugates Immunoglobulins Maleimides Single-Domain Antibodies Tandem Mass Spectrometry

BACKGROUND: Intrabodies enable targeting of proteins in live cells, but generating specific intrabodies against the thousands of proteins in a proteome poses a challenge. We leverage the widespread availability of fluorescently...

cell signalling Endoplasmic Reticulum Fluorescence microscopy Fluorescent protein GFP intrabody membrane contact site Mitochondria Nanobody RFP Animals COS Cells Chlorocebus aethiops Green Fluorescent Proteins HeLa Cells Humans Luminescent Proteins Plasmids Signal Transduction Single-Domain Antibodies

Protein misfolding and aggregation is the hallmark of numerous human disorders, including Alzheimer's disease. This process involves the formation of transient and heterogeneous soluble oligomers, some of which are highly...

Alzheimer’s disease Amyloid protein aggregation protein design Alzheimer Disease Amyloid beta-Peptides Animals Antibodies Antibody Specificity Caenorhabditis elegans Disease Models Animal Epitopes Hippocampus Mice Protein Aggregates Protein Binding Protein Conformation Single-Domain Antibodies

In silico antibody discovery is emerging as a viable alternative to traditional in vivo and in vitro approaches. Many challenges, however, remain open to enabling the properties of designed antibodies to match those produced by...

antibody design antibody engineering metadynamics Molecular dynamics protein design Antibodies Complementarity Determining Regions Entropy Molecular Conformation Single-Domain Antibodies

Latent human cytomegalovirus (HCMV) infection is characterized by limited gene expression, making latent HCMV infections refractory to current treatments targeting viral replication. However, reactivation of latent HCMV in...

Cells Cultured Cytomegalovirus Cytomegalovirus Infections Gene Expression Genes Immediate-Early Humans Lipopolysaccharide Receptors Monocytes Receptors Chemokine Signal Transduction Single-Domain Antibodies T-Lymphocytes Cytotoxic Viral Proteins Virus Activation Virus Latency

Intracellular deposits of α-synuclein in the form of Lewy bodies are major hallmarks of Parkinson's disease (PD) and a range of related neurodegenerative disorders. Post-translational modifications (PTMs) of α-synuclein are...

Parkinson's Disease Isothermal titration calorimetry nanobody) Nuclear Magnetic Resonance Phosphorylation protein misfolding single-domain antibody (sdAb Surface Plasmon Resonance α-synuclein Autopsy Binding Sites Brain Humans Parkinson Disease Phosphorylation Protein Binding Protein Processing Post-Translational Serine Single-Domain Antibodies Tyrosine ALPHA-SYNUCLEIN

Amino Acid Substitution Antigen-Antibody Reactions Binding Sites Crystallography X-Ray Escherichia coli Proteins Galactose Glycine Hydrogen Bonding Kinetics Models Molecular Monosaccharide Transport Proteins Mutation Missense Point Mutation Protein Binding Protein Conformation Protein Stability Single-Domain Antibodies Structure-Activity Relationship Symporters Thiogalactosides Tryptophan

Camelid single-domain antibodies, also known as nanobodies, can be readily isolated from naïve libraries for specific targets but often bind too weakly to their targets to be immediately useful. Laboratory-based genetic...

covid-19 affinity biophysics computational tool Electron microscopy Antibodies Neutralizing Antibodies Viral Antibody Affinity Cryoelectron Microscopy Entropy Genetic Engineering Humans Protein Binding Protein Domains SARS-CoV-2 Single-Domain Antibodies Spike Glycoprotein Coronavirus

T-Lymphocytes Cytotoxic Monocytes Cells Cultured Humans Cytomegalovirus Cytomegalovirus Infections Receptors Chemokine Viral Proteins Virus Latency Virus Activation Signal Transduction Gene Expression Genes Immediate-Early Single-Domain Antibodies Lipopolysaccharide Receptors

A complex conformational energy landscape determines G-protein-coupled receptor (GPCR) signalling via intracellular binding partners (IBPs), e.g., Gs and β-arrestin. Using 13C methyl methionine NMR for the β1-adrenergic...

Adrenergic beta-2 Receptor Agonists Animals Crystallography X-Ray GTP-Binding Protein alpha Subunits Gs Ligands Nuclear Magnetic Resonance Biomolecular Protein Conformation Receptors Adrenergic beta-2 Sf9 Cells Signal Transduction Single-Domain Antibodies Spodoptera

Post-translational protein-protein conjugation produces bioconjugates that are unavailable via genetic fusion approaches. A method for preparing protein-protein conjugates using π-clamp-mediated cysteine arylation with...

covid-19 Cysteine Humans Proteins SARS-CoV-2 Single-Domain Antibodies

Amyloid beta-Peptides Animals Astrocytes Calcium Carbocyanines Cell Membrane Cells Cultured Clusterin Microscopy Fluorescence Neurons Peptide Fragments Photobleaching Protein Aggregates Rats Single-Domain Antibodies

One potential therapeutic strategy for Alzheimer's disease (AD) is to use antibodies that bind to small soluble protein aggregates to reduce their toxic effects. However, these therapies are rarely tested in human CSF before...

Alzheimer’s disease Antibodies beta amyloid calcium influx cerebrospinal fluid Clusterin fluorescence measurements neurodegenerative conditions OLIGOMERS single molecule imaging Aged Alzheimer Disease Amino Acid Sequence Amyloid beta-Peptides Animals Antibodies Monoclonal Humanized Astrocytes Biological Assay Calcium cerebrospinal fluid Clusterin Culture Media Cytoplasmic Vesicles Female Humans Ion Transport Male Middle Aged Peptide Fragments Primary Cell Culture Protein Aggregates Rats Single-Domain Antibodies

Nanobodies are a class of antigen-binding protein derived from camelids that achieve comparable binding affinities and specificities to classical antibodies, despite comprising only a single 15 kDa variable domain. Their reduced...

HcAb VH VHH Antibody camelid FRAMEWORK heavy chain antibody loop single domain antibody Antibodies Antibody Specificity Binding Sites Antibody Models Molecular Protein Conformation Sequence Alignment Single-Domain Antibodies Structure-Activity Relationship

antibody maturation chemical mutagenesis non-natural amino acids protein aggregation Alzheimer Disease Amino Acids Humans Protein Processing Post-Translational Single-Domain Antibodies Structure-Activity Relationship