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This article describes the implementation of real-space refinement in the phenix.real_space_refine program from the PHENIX suite. The use of a simplified refinement target function enables very fast calculation, which in turn...

PHENIX atomic-centered targets cryo-EM Crystallography map interpolation real-space refinement Animals Computer Simulation Cryoelectron Microscopy Crystallography Databases Protein Humans Macromolecular Substances Models Molecular Software TRPV Cation Channels Validation Studies as Topic

Administration Oral Adult Aged Area Under Curve Benzimidazoles Case-Control Studies Double-Blind Method Drug Administration Schedule Female Food-Drug Interactions Half-Life Heart Failure Humans Male Spiro Compounds TRPV Cation Channels

Animals Animals Newborn Calcitonin Gene-Related Peptide Calcium Signaling Ganglia Spinal Hot Temperature Immunohistochemistry In Situ Hybridization In Vitro Techniques Ion Channel Gating Nerve Growth Factors Nociceptors Protein Kinase C-epsilon Protein Transport Rats Rats Sprague-Dawley Receptor PAR-1 Receptors Thrombin TRPV Cation Channels Thrombin Up-Regulation

BACKGROUND: Host factors required for retroviral infection are potential targets for the modulation of diseases caused by retroviruses. During the retroviral life cycle, numerous cellular factors interact with the virus and play...

Adenosine Triphosphatases Animals Calcium Channels Cells Cultured Cloning Molecular DNA Helicases Embryonic Stem Cells Genes Recessive Genetic Markers Genetic Vectors Immunity Innate Integrases Leukemia Virus Murine Mice Mutagenesis Insertional RecQ Helicases Receptors Virus TRPV Cation Channels

Knee neuron osteochondral defect Pain sheep model Animals Capsaicin Female Ganglia Spinal Humans Neurons Pain Sheep TRPV Cation Channels

calcium imaging capsaicin receptor Electrophysiology Hyperalgesia Nerve Growth Factor nociceptor development receptor kinase signaling sensitization transcriptome assembly Animals Ganglia Spinal HEK293 Cells Humans Ion Channel Gating Mole Rats Nerve Growth Factor Neurons Nociceptors Pain Protein Domains Proteomics Receptor trkA TRPV Cation Channels

Transient receptor potential vanilloid 2 (TRPV2) is involved in many critical physiological and pathophysiological processes, making it a promising drug target. Here we present cryo-electron microscopy (cryo-EM) structures of...

Animals Binding Sites Cryoelectron Microscopy Protein Domains Rats TRPV Cation Channels

placental development Primary trophoblast cells TRP channels trophoblast stem cells Animals Calcium Channels Calcium Signaling Cell Differentiation Cell Proliferation Female Gene Expression Regulation Mice Mice Inbred C57BL Mouse embryonic stem cells Placenta Placentation Pregnancy Stem Cells TRPV Cation Channels Transient Receptor Potential Channels Trophoblasts

Digging behavior dorsal root ganglion neurons Inflammation Joint pain sensitization TRPV1 Animals Anti-Inflammatory Agents Non-Steroidal Arthralgia Capsaicin Cells Cultured Disease Models Animal Female Freund's Adjuvant Ganglia Spinal Hindlimb Inflammation Isoquinolines Mice Inbred C57BL Motor Activity Receptor trkA Sensory Receptor Cells TRPV Cation Channels Urea gamma-Aminobutyric Acid

BACKGROUND: Morphine and its derivatives are key drugs in pain control. Despite its well-known analgesic properties morphine at high concentrations may be proalgesic. Particularly, short-lasting painful sensations have been...

Acrolein Animals Bradykinin Calcitonin Gene-Related Peptide Calcium Signaling Capsaicin Dose-Response Relationship Drug Extremities Ganglia Spinal Hot Temperature Humans Ion Channel Gating Mice Morphine Naloxone Neurons Skin TRPA1 Cation Channel TRPV Cation Channels Transient Receptor Potential Channels

Visceral pain is a leading cause of morbidity in gastrointestinal diseases, which is exacerbated by the gut-related side-effects of many analgesics. New treatments are needed and further understanding of the mediators and...

TRPV1 Capsaicin inflammatory bowel disease Irritable Bowel Syndrome nociception p38 MAPK tumour necrosis factor-alpha visceral pain Animals Capsaicin Ganglia Spinal Mice Nociceptors Receptors Tumor Necrosis Factor Type I TRPV Cation Channels Tumor Necrosis Factor-alpha visceral pain p38 Mitogen-Activated Protein Kinases

Post-infectious irritable bowel syndrome (PI-IBS) is a common gastrointestinal disorder characterized by persistent abdominal pain despite recovery from acute gastroenteritis. The underlying mechanisms are unclear, although...

Adult Animals Capsaicin Case-Control Studies Colon Cytokines Female Ganglia Spinal Gastroenteritis Humans Intestinal Mucosa Irritable Bowel Syndrome Male Mice Mice Inbred C57BL Middle Aged Neurons Receptors Histamine H1 Signal Transduction TRPV Cation Channels

OBJECTIVE: The development of effective visceral analgesics free of deleterious gut-specific side effects is a priority. We aimed to develop a reproducible methodology to study visceral nociception in human tissue that could aid...

Abdominal Pain Electrophysiology NEUROGASTROENTEROLOGY visceral nociception VISCERAL SENSITIVITY Adenosine Triphosphate Anti-Inflammatory Agents Non-Steroidal Bradykinin Bradykinin Receptor Antagonists Drug Evaluation Preclinical Gastrointestinal Agents Humans Indoles Intestines Morpholines Nociceptors Physical Stimulation Pyrroles Serotonin Receptor Agonists TRPV Cation Channels Tissue Culture Techniques

We report the design, synthesis and biological evaluation of natural product-drug conjugates for treatment of prostate cancers over-expressing the transient receptor potential vanilloid 1 (TRPV1) channel. We validate the...

Cancer chemical biology Drug delivery Ligand-gated ion channel Small molecule–drug conjugates Antineoplastic Agents Biological Products Capsaicin Cell Line Tumor Cell Survival Gene Expression Humans Ligands Small Molecule Libraries TRPV Cation Channels Temozolomide