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Mechanical design principles of a mitotic spindle.
An organised spindle is crucial to the fidelity of chromosome segregation, but the relationship between spindle structure and function is not well understood in any cell type. The anaphase B spindle in fission yeast has a...
A RhoA-FRET Biosensor Mouse for Intravital Imaging in Normal Tissue Homeostasis and Disease Contexts.
Max Nobis, David Herrmann, Sean C Warren, Shereen Kadir, Wilfred Leung, Monica Killen, Astrid Magenau, David Stevenson, Morghan C Lucas, Nadine Reischmann, Claire Vennin, James RW Conway, Alice Boulghourjian, Anaiis Zaratzian, Andrew M Law, David Gallego-Ortega, Christopher J Ormandy, Stacey N Walters, Shane T Grey, Jacqueline Bailey, Tatyana Chtanova, Julian MW Quinn, Paul A Baldock, Peter I Croucher, Juliane P Schwarz, Agata Mrowinska, Lei Zhang, Herbert Herzog, Andrius Masedunskas, Edna C Hardeman, Peter W Gunning, Gonzalo Del Monte-Nieto, Richard P Harvey, Michael S Samuel, Marina Pajic, Ewan J McGhee, Anna-Karin E Johnsson, Owen J Sansom, Heidi CE Welch, Jennifer P Morton, Douglas Strathdee, Kurt I Anderson, Paul Timpson
Jun 27, 2019
The small GTPase RhoA is involved in a variety of fundamental processes in normal tissue. Spatiotemporal control of RhoA is thought to govern mechanosensing, growth, and motility of cells, while its deregulation is associated...
FLIM-FRET actin Biosensors Breast Cancer Cell Biology development immunology intravital imaging pancreatic cancer small GTPase RhoA Animals Antineoplastic Agents Biosensing Techniques Bone and Bones Cell Movement Dasatinib Erlotinib Hydrochloride Female Fluorescence Resonance Energy Transfer Gene Expression Regulation Intestine Small Intravital Microscopy Mammary Glands Animal Mammary Neoplasms Experimental Mechanotransduction Cellular Mice Mice Transgenic Neutrophils Osteocytes Pancreatic Neoplasms Time-Lapse Imaging rho GTP-Binding Proteins rhoA GTP-Binding Protein
Radially patterned cell behaviours during tube budding from an epithelium.
The budding of tubular organs from flat epithelial sheets is a vital morphogenetic process. Cell behaviours that drive such processes are only starting to be unraveled. Using live-imaging and novel morphometric methods, we show...
D. melanogaster Cell Biology Cell Shape developmental biology Epithelia Morphogenesis Organogenesis quantitative analysis radial polarity Animals Cell Polarity Cell Shape Drosophila melanogaster Embryo Nonmammalian Epithelial Cells Epithelium Fluorescence Imaging Three-Dimensional Morphogenesis Mutation Myosin Type II Salivary Glands Time-Lapse Imaging
Escherichia coli can survive stress by noisy growth modulation.
Om Patange, Christian Schwall, Matt Jones, Casandra Villava, Douglas A Griffith, Andrew Phillips, James CW Locke
Feb 05, 2019
Gene expression can be noisy, as can the growth of single cells. Such cell-to-cell variation has been implicated in survival strategies for bacterial populations. However, it remains unclear how single cells couple gene...
Subcellular localization determines the stability and axon protective capacity of axon survival factor Nmnat2.
Axons require a constant supply of the labile axon survival factor Nmnat2 from their cell bodies to avoid spontaneous axon degeneration. Here we investigate the mechanism of fast axonal transport of Nmnat2 and its site of action...
Amino Acid Motifs Animals Axons Cell Survival Cells Cultured Exons Golgi Apparatus Half-Life Intracellular Membranes Lipoylation Mice Mice Inbred C57BL Neurons Nicotinamide-Nucleotide Adenylyltransferase Primary Cell Culture Protein Multimerization Protein Stability Protein Transport Single-Cell Analysis Time-Lapse Imaging Transport Vesicles Ubiquitination
Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene.
Linda S Forero-Quintero, William Raymond, Tetsuya Handa, Matthew N Saxton, Tatsuya Morisaki, Hiroshi Kimura, Edouard Bertrand, Brian Munsky, Timothy J Stasevich
Jun 27, 2021
The carboxyl-terminal domain of RNA polymerase II (RNAP2) is phosphorylated during transcription in eukaryotic cells. While residue-specific phosphorylation has been mapped with exquisite spatial resolution along the 1D genome...
Using Tetracysteine-Tagged TDP-43 with a Biarsenical Dye To Monitor Real-Time Trafficking in a Cell Model of Amyotrophic Lateral Sclerosis.
Janice SW Ng, Maya A Hanspal, Naunehal S Matharu, Teresa P Barros, Elin K Esbjörner, Mark R Wilson, Justin J Yerbury, Christopher M Dobson, Janet R Kumita
Sep 09, 2019
TAR DNA-binding protein 43 (TDP-43) has been identified as the major constituent of the proteinaceous inclusions that are characteristic of most forms of amyotrophic lateral sclerosis (ALS) and ubiquitin positive frontotemporal...
Amino Acid Sequence Amyotrophic Lateral Sclerosis Cell Line Tumor Cell Nucleus Cysteine Cytosol DNA-Binding Proteins Electroporation Fluoresceins Fluorescent Dyes Frontotemporal Lobar Degeneration Humans Kinetics Models Biological Optical Imaging Organometallic Compounds Protein Transport Sequence Tagged Sites Time-Lapse Imaging Transfection
CytoCensus, mapping cell identity and division in tissues and organs using machine learning.
Martin Hailstone, Dominic Waithe, Tamsin J Samuels, Lu Yang, Ita Costello, Yoav Arava, Elizabeth Robertson, Richard M Parton, Ilan Davis
Jun 08, 2020
A major challenge in cell and developmental biology is the automated identification and quantitation of cells in complex multilayered tissues. We developed CytoCensus: an easily deployed implementation of supervised machine...
3D cell detection 4D image analysis D. melanogaster Cell Biology developmental biology ex vivo culture Live imaging MOUSE Neural Stem Cells Zebrafish Animals Animals Genetically Modified Automation Brain Cell Division Drosophila melanogaster Embryo Mammalian Female Image Processing Computer-Assisted Larva Machine Learning Male Mice Microscopy Video Mutation Organoids Phenotype Reproducibility of Results Retina Time Factors Time-Lapse Imaging Tissue Culture Techniques Zebrafish
Single-molecule visualization of DNA G-quadruplex formation in live cells.
Marco Di Antonio, Aleks Ponjavic, Antanas Radzevičius, Rohan T Ranasinghe, Marco Catalano, Xiaoyun Zhang, Jiazhen Shen, Lisa-Maria Needham, Steven F Lee, David Klenerman, Shankar Balasubramanian
Jun 23, 2020
High proliferation and delamination during skin epidermal stratification.
Mareike Damen, Lisa Wirtz, Ekaterina Soroka, Houda Khatif, Christian Kukat, Benjamin D Simons, Hisham Bazzi
Jun 29, 2021
The development of complex stratified epithelial barriers in mammals is initiated from single-layered epithelia. How stratification is initiated and fueled are still open questions. Previous studies on skin epidermal...
epidermis Hair Follicle Centrosome Keratinocytes Animals Mice Knockout Humans Mice Ubiquitin Thiolesterase Cell Differentiation Cell Proliferation Models Biological Adolescent Adult Aged Middle Aged Child Child Preschool Female Male Tumor Suppressor Protein p53 Embryo Mammalian Skin Physiological Phenomena Young Adult Time-Lapse Imaging Asymmetric cell division Spindle Apparatus Tumor Suppressor p53-Binding Protein 1
Published by: Nature Communications
Drosophila Dendritic Arborisation Neurons
Neuronal dendrites receive, integrate, and process numerous inputs and therefore serve as the neuron’s “antennae”. Dendrites display extreme morphological diversity across different neuronal classes to match the neuron’s...
Drosophila Dendritic Arborisation Neurons
Neuronal dendrites receive, integrate, and process numerous inputs and therefore serve as the neuron's "antennae". Dendrites display extreme morphological diversity across different neuronal classes to match the neuron's...
Challenges of analysing stochastic gene expression in bacteria using single-cell time-lapse experiments.
Stochastic gene expression causes phenotypic heterogeneity in a population of genetically identical bacterial cells. Such non-genetic heterogeneity can have important consequences for the population fitness, and therefore cells...
Stoyana Alexandrova, Tuzer Kalkan, Peter Humphreys, Andrew Riddell, Roberta Scognamiglio, Andreas Trumpp, Jennifer Nichols
Nov 16, 2015
Inhibitory neuron migration and IPL formation in the developing zebrafish retina.
Human keratinocytes have two interconvertible modes of proliferation.
Amit Roshan, Kasumi Murai, Joanna Fowler, Benjamin D Simons, Varvara Nikolaidou-Neokosmidou, Philip H Jones
Nov 23, 2015
Expression and knockdown of zebrafish folliculin suggests requirement for embryonic brain morphogenesis.
Emma J Kenyon, Monique NH Luijten, Harmeet Gill, Nan Li, Matthew Rawlings, James C Bull, Yavor Hadzhiev, Maurice AM van Steensel, Eamonn Maher, Ferenc Mueller
Jul 21, 2016
Birt-Hogg-Dubé syndrome (BHD) Brain Cell Cycle folliculin Zebrafish flcn zFucci Animals Brain Cell Cycle Gene Expression Regulation Developmental Gene Knockdown Techniques Humans Proto-Oncogene Proteins Retina Somites Time-Lapse Imaging Tumor Suppressor Proteins Up-Regulation Zebrafish Zebrafish Proteins
High proliferation and delamination during skin epidermal stratification.
Mareike Damen, Lisa Wirtz, Ekaterina Soroka, Houda Khatif, Christian Kukat, Benjamin D Simons, Hisham Bazzi
Jul 05, 2021
Adolescent Adult Aged Animals Asymmetric cell division Cell Differentiation Cell Proliferation Centrosome Child Child Preschool Embryo Mammalian epidermis Female Hair Follicle Humans Keratinocytes Male Mice Mice Knockout Middle Aged Models Biological Skin Physiological Phenomena Spindle Apparatus Time-Lapse Imaging Tumor Suppressor Protein p53 Tumor Suppressor p53-Binding Protein 1 Ubiquitin Thiolesterase Young Adult
Oncogenic KRAS triggers MAPK-dependent errors in mitosis and MYC-dependent sensitivity to anti-mitotic agents.
Active Transport Cell Nucleus Antineoplastic Agents Cell Line Tumor Cell Nucleus Chromosome Segregation HCT116 Cells HeLa Cells Humans Microscopy Confocal Mitogen-Activated Protein Kinases Mitosis Mutation Proto-Oncogene Proteins c-myc Proto-Oncogene Proteins p21(ras) RNA Interference Time-Lapse Imaging
Stochastic pulsing of gene expression enables the generation of spatial patterns in Bacillus subtilis biofilms.
Stochastic pulsing of gene expression can generate phenotypic diversity in a genetically identical population of cells, but it is unclear whether it has a role in the development of multicellular systems. Here, we show how...
The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis.
Luisa Capalbo, Zuni I Bassi, Marco Geymonat, Sofia Todesca, Liviu Copoiu, Anton J Enright, Giuliano Callaini, Maria Giovanna Riparbelli, Lu Yu, Jyoti S Choudhary, Enrico Ferrero, Sally Wheatley, Max E Douglas, Masanori Mishima, Pier Paolo D'Avino
Aug 29, 2019
The midbody is an organelle assembled at the intercellular bridge between the two daughter cells at the end of mitosis. It controls the final separation of the daughter cells and has been involved in cell fate, polarity, tissue...
Aurora Kinase B Binding Sites Cytokinesis HeLa Cells Humans Intravital Microscopy Microtubule-Associated Proteins Microtubules Mitosis Mutagenesis Site-Directed Myosin-Light-Chain Phosphatase Phosphorylation Protein Interaction Maps Protein Phosphatase 1 RNA Small Interfering Spindle Apparatus Time-Lapse Imaging
Stable centrosomal roots disentangle to allow interphase centriole independence.
Robert Mahen
Jul 25, 2018
Claire M Lye, Guy B Blanchard, Huw W Naylor, Leila Muresan, Jan Huisken, Richard J Adams, Bénédicte Sanson
Oct 07, 2015
Animals Biomarkers Cell Shape Cell Size Drosophila Drosophila Proteins Embryo Nonmammalian Endoderm Gastrulation Green Fluorescent Proteins Mechanical Phenomena Membrane Fusion Proteins Microscopy Electron Scanning Microscopy Video Models Anatomic Morphogenesis Mutation Recombinant Fusion Proteins Rheology Time-Lapse Imaging Twist-Related Protein 1
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