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Computer simulations reveal motor properties generating stable antiparallel microtubule interactions.
François Nédélec
Jul 30, 2020
An aster of microtubules is a set of flexible polar filaments with dynamic plus ends that irradiate from a common location at which the minus ends of the filaments are found. Processive soluble oligomeric motor complexes can...
The E3 ubiquitin ligase skp2 regulates neural differentiation independent from the cell cycle.
Hector Boix-Perales, Ian Horan, Helen Wise, Horng-Ru Lin, Li-Chiou Chuang, P Renee Yew, Anna Philpott
Jun 14, 2011
Animals Cell Cycle Cell Cycle Proteins Cell Differentiation Cell Proliferation Central Nervous System Gene Expression Regulation Developmental Gene Expression Regulation Enzymologic Models Animal Neural Tube Neurons S-Phase Kinase-Associated Proteins Stem Cells Ubiquitin-Protein Ligases Xenopus Proteins Xenopus laevis
Cytoplasmic polyadenylation and cytoplasmic polyadenylation element-dependent mRNA regulation are involved in Xenopus retinal axon development.
Andrew C Lin, Chin Lik Tan, Chien-Ling Lin, Laure Strochlic, Yi-Shuian Huang, Joel D Richter, Christine E Holt
Jun 16, 2011
Animals Axons Base Sequence Cytoplasm Fluorescent Antibody Technique Gene Expression Regulation In Situ Hybridization Molecular Sequence Data Plasmids Polyadenylation Protein Biosynthesis RNA Messenger Retinal Ganglion Cells Reverse Transcriptase Polymerase Chain Reaction Transcription Factors Xenopus Xenopus Proteins mRNA Cleavage and Polyadenylation Factors
Activity-dependent alteration of early myelin ensheathment in a developing sensory circuit.
Zahraa Chorghay, David MacFarquhar, Vanessa J Li, Sarah Aufmkolk, Anne Schohl, Paul W Wiseman, Ragnhildur Thóra Káradóttir, Edward S Ruthazer
Oct 13, 2021
Myelination allows for the regulation of conduction velocity, affecting the precise timing of neuronal inputs important for the development and function of brain circuits. In turn, myelination may be altered by changes in...
Structural and calorimetric studies demonstrate that the hepatocyte nuclear factor 1β (HNF1β) transcription factor is imported into the nucleus via a monopartite NLS sequence.
Hepatocyte nuclear factor-1β (HNF1β) Importin-α Isothermal titration calorimetry Nuclear import pathway Nuclear localisation signal sequence (NLS) Site-directed mutagenesis X-ray crystallography Active Transport Cell Nucleus Animals Binding Sites Cell Line Tumor Cell Nucleus Crystallography X-Ray HEK293 Cells Hepatocyte Nuclear Factor 1-beta Humans Mice Models Molecular Nuclear Localization Signals Protein Binding Protein Conformation alpha-Helical Sequence Analysis Protein Xenopus Proteins Xenopus laevis alpha Karyopherins
Hermes Regulates Axon Sorting in the Optic Tract by Post-Trancriptional Regulation of Neuropilin 1.
RNA-binding protein axon sorting topography visual system Animals Axons Embryo Nonmammalian Gene Knockdown Techniques Growth Cones Neuropilin-1 Protein Processing Post-Translational RNA-Binding Proteins Retinal Ganglion Cells Semaphorin-3A Superior Colliculi Visual Pathways Xenopus Proteins Xenopus laevis Zebrafish
CPEB and miR-15/16 Co-Regulate Translation of Cyclin E1 mRNA during Xenopus Oocyte Maturation.
Animals Base Sequence Cyclin E Female Meiosis MicroRNAs Models Biological Molecular Sequence Data Oocytes Oogenesis Protein Biosynthesis RNA 3' Polyadenylation Signals RNA Messenger RNA-Induced Silencing Complex Transcription Factors Xenopus Proteins Xenopus laevis mRNA Cleavage and Polyadenylation Factors
Amputation-induced reactive oxygen species are required for successful Xenopus tadpole tail regeneration.
Nick R Love, Yaoyao Chen, Shoko Ishibashi, Paraskevi Kritsiligkou, Robert Lea, Yvette Koh, Jennifer L Gallop, Karel Dorey, Enrique Amaya
Jun 22, 2020
Understanding the molecular mechanisms that promote successful tissue regeneration is critical for continued advancements in regenerative medicine. Vertebrate amphibian tadpoles of the species Xenopus laevis and Xenopus...
Amputation Surgical Animals Animals Genetically Modified antioxidants Cell Proliferation Enzyme Inhibitors Fibroblast Growth Factors Gene Expression Regulation Hydrogen Peroxide Larva NADPH Oxidases Oligonucleotides Antisense Reactive Oxygen Species Regeneration Tail Time Factors Wnt Proteins Wnt Signaling Pathway Xenopus Proteins Xenopus laevis beta Catenin
The F-box protein Cdc4/Fbxw7 is a novel regulator of neural crest development in Xenopus laevis.
Alexandra D Almeida, Helen M Wise, Christopher J Hindley, Michael K Slevin, Rebecca S Hartley, Anna Philpott
Jun 13, 2011
BACKGROUND: The neural crest is a unique population of cells that arise in the vertebrate ectoderm at the neural plate border after which they migrate extensively throughout the embryo, giving rise to a wide range of...
Animals Blotting Western Cell Differentiation Cell Proliferation Cyclin E DNA Complementary F-Box Proteins F-Box-WD Repeat-Containing Protein 7 Gene Expression Regulation Developmental In Situ Hybridization Microinjections Neural Crest Plasmids Point Mutation Polymerase Chain Reaction Protein Isoforms RNA Messenger Sequence Deletion Ubiquitin Xenopus Proteins Xenopus laevis
Multi-site phosphorylation regulates NeuroD4 activity during primary neurogenesis
Joanna R Watson, Helen M Fox, Daniel Nietlispach, Jennifer L Gallop, Darerca Owen, Helen R Mott
May 05, 2016
BAR domain Cdc42 CIP4 FBP17 TOCA1 WASP actin Endocytosis Nuclear Magnetic Resonance (NMR) Protein-Protein Interaction Animals Carrier Proteins Fatty Acid-Binding Proteins Humans Monomeric GTP-Binding Proteins Protein Binding Protein Domains Protein Structure Quaternary Wiskott-Aldrich Syndrome Protein Neuronal Xenopus Proteins Xenopus laevis
(1)H, (13)C and (15)N resonance assignments of the Cdc42-binding domain of TOCA1.
Regulation of chemotropic guidance of nerve growth cones by microRNA.
Liang Han, Zhexing Wen, Rachel C Lynn, Marie-Laure Baudet, Christine E Holt, Yukio Sasaki, Gary J Bassell, James Q Zheng
Nov 16, 2011
A computational model predicts Xenopus meiotic spindle organization.
The metaphase spindle is a dynamic bipolar structure crucial for proper chromosome segregation, but how microtubules (MTs) are organized within the bipolar architecture remains controversial. To explore MT organization along the...
Calculating the Degradation Rate of Individual Proteins Using Xenopus Extract Systems.
The Xenopus extract system has been used extensively as a simple, quick, and robust method for assessing the stability of proteins against proteasomal degradation. In this protocol, methods are provided for assessing the...
N-terminal phosphorylation of xHes1 controls inhibition of primary neurogenesis in Xenopus.
Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons.
Jean-Michel Cioni, Julie Qiaojin Lin, Anne V Holtermann, Max Koppers, Maximilian AH Jakobs, Afnan Azizi, Benita Turner-Bridger, Toshiaki Shigeoka, Kristian Franze, William A Harris, Christine E Holt
Dec 17, 2018
Local translation regulates the axonal proteome, playing an important role in neuronal wiring and axon maintenance. How axonal mRNAs are localized to specific subcellular sites for translation, however, is not understood. Here...
Charcot-Marie-Tooth type 2B RNA trafficking axon maintenance endosome local protein synthesis lysosome mRNA translation Mitochondria Retinal ganglion cell Animals Axons Endosomes Mitochondria Protein Biosynthesis RNA RNA Messenger Retinal Ganglion Cells Ribosomes Xenopus Proteins Xenopus laevis rab GTP-Binding Proteins rab7 GTP-Binding Proteins
Chk1 Inhibition of the Replication Factor Drf1 Guarantees Cell-Cycle Elongation at the Xenopus laevis Mid-blastula Transition
DDK Drf1 MBT Xenopus laevis Cell Cycle checkpoint chk1 midblastula transition replication initiation Amino Acid Sequence Animals Blastula Cell Cycle Checkpoint Kinase 1 Chromosomal Proteins Non-Histone DNA Replication Down-Regulation Embryo Nonmammalian Embryonic Development Gene Expression Regulation Developmental Phosphorylation Proteolysis SKP Cullin F-Box Protein Ligases Xenopus Proteins Xenopus laevis beta-Transducin Repeat-Containing Proteins
ESCRT-II controls retinal axon growth by regulating DCC receptor levels and local protein synthesis.
DCC ESCRT Netrin-1 Axon guidance Endocytosis protein synthesis Animals Axons Endocytosis Endosomal Sorting Complexes Required for Transport Endosomes Gene Knockdown Techniques Growth Cones Nerve Growth Factors Phenotype Protein Biosynthesis RNA Messenger Receptors Cell Surface Retina Retinal Ganglion Cells Tumor Suppressor Proteins Xenopus Proteins Xenopus laevis
Smarcal1-Mediated Fork Reversal Triggers Mre11-Dependent Degradation of Nascent DNA in the Absence of Brca2 and Stable Rad51 Nucleofilaments.
Arun Mouli Kolinjivadi, Vincenzo Sannino, Anna De Antoni, Karina Zadorozhny, Mairi Kilkenny, Hervé Técher, Giorgio Baldi, Rong Shen, Alberto Ciccia, Luca Pellegrini, Lumir Krejci, Vincenzo Costanzo
Sep 03, 2019
BRCA2 DNA Replication Mre11 RAD51 Xenopus laevis fork protection Animals BRCA2 Protein Binding Sites DNA DNA Helicases DNA Polymerase I DNA Polymerase III DNA Replication DNA-Binding Proteins Endodeoxyribonucleases Exodeoxyribonucleases Female Genomic Instability Humans MRE11 Homologue Protein Male Mutation Protein Binding Rad51 Recombinase Replication Origin Saccharomyces cerevisiae Proteins Time Factors Xenopus Proteins Xenopus laevis
Ascl1 phospho-status regulates neuronal differentiation in a Xenopus developmental model of neuroblastoma.
Ascl1 Cell Cycle development DIFFERENTIATION Neuroblastoma Xenopus Adrenergic Neurons Animals Basic Helix-Loop-Helix Transcription Factors Biomarkers Cell Differentiation Cell Movement Cyclin-Dependent Kinase Inhibitor p27 Cyclin-Dependent Kinases Disease Models Animal Embryo Nonmammalian Gene Knockdown Techniques Morpholinos Nerve Tissue Proteins Neural Crest Neuroblastoma Neurogenesis Neurons Phosphorylation Proto-Oncogene Proteins c-myc Xenopus Proteins Xenopus laevis
Single-molecule analysis of endogenous β-actin mRNA trafficking reveals a mechanism for compartmentalized mRNA localization in axons.
Benita Turner-Bridger, Maximillian Jakobs, Leila Muresan, Hovy Ho-Wai Wong, Kristian Franze, William A Harris, Christine E Holt
Nov 22, 2018
During embryonic nervous system assembly, mRNA localization is precisely regulated in growing axons, affording subcellular autonomy by allowing controlled protein expression in space and time. Different sets of mRNAs exhibit...
Assessing Ubiquitylation of Individual Proteins Using Xenopus Extract Systems.
Xenopus extract systems have been used to study ubiquitylation of proteins, and to uncover some of the fundamental processes of the ubiquitylation pathway itself. They provide a simple, quick, and robust method for studying...
Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring.
Roberta Cagnetta, Hovy Ho-Wai Wong, Christian K Frese, Giovanna R Mallucci, Jeroen Krijgsveld, Christine E Holt
Dec 14, 2018
Local translation is rapidly regulated by extrinsic signals during neural wiring, but its control mechanisms remain elusive. Here we show that the extracellular cue Sema3A induces an initial burst in local translation that...
PERK Sema3A axon axon branching Axon guidance eIF2B eIF2α local translation Retinal ganglion cell Unfolded Protein Response Animals Axons Eukaryotic Initiation Factor-2 Eukaryotic Initiation Factor-2B Female Male Neurogenesis Phosphorylation Protein Interaction Maps Proteomics Retinal Ganglion Cells Semaphorin-3A Signal Transduction Tissue Culture Techniques Xenopus Proteins Xenopus laevis eIF-2 Kinase
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