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Disease modelling in human organoids.
The past decade has seen an explosion in the field of in vitro disease modelling, in particular the development of organoids. These self-organizing tissues derived from stem cells provide a unique system to examine mechanisms...
Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation.
Laura Broutier, Amanda Andersson-Rolf, Christopher J Hindley, Sylvia F Boj, Hans Clevers, Bon-Kyoung Koo, Meritxell Huch
Jun 28, 2016
Lgr5+ stem and progenitor cells reside at the apex of a heterogeneous embryonic hepatoblast pool.
Nicole Prior, Christopher J Hindley, Fabian Rost, Elena Meléndez, Winnie WY Lau, Berthold Göttgens, Steffen Rulands, Benjamin D Simons, Meritxell Huch
May 17, 2019
Bipotent Hepatoblast Lgr5 Liver development Liver stem/progenitor cells Organoid Alleles Animals Base Sequence Cell Count Cell Culture Techniques Cell Differentiation Cell Lineage Cells Cultured Embryonic Development Epithelial Cells Female Gene Expression Regulation Developmental Hepatocytes Homeostasis Liver Male Mice Microscopy Confocal Receptors G-Protein-Coupled Stem Cells
Neurogenin3 phosphorylation controls reprogramming efficiency of pancreatic ductal organoids into endocrine cells.
Roberta Azzarelli, Steffen Rulands, Sonia Nestorowa, John Davies, Sara Campinoti, Sébastien Gillotin, Paola Bonfanti, Berthold Göttgens, Meritxell Huch, Benjamin Simons, Anna Philpott
Dec 03, 2018
RNF43/ZNRF3 loss predisposes to hepatocellular-carcinoma by impairing liver regeneration and altering the liver lipid metabolic ground-state.
Germán Belenguer, Gianmarco Mastrogiovanni, Clare Pacini, Zoe Hall, Anna M Dowbaj, Robert Arnes-Benito, Aleksandra Sljukic, Nicole Prior, Sofia Kakava, Charles Bradshaw, Susan Davies, Michele Vacca, Kourosh Saeb-Parsy, Bon-Kyoung Koo, Meritxell Huch
Jan 28, 2022
RNF43/ZNRF3 negatively regulate WNT signalling. Both genes are mutated in several types of cancers, however, their contribution to liver disease is unknown. Here we describe that hepatocyte-specific loss of Rnf43/Znrf3 results...
RNF43/ZNRF3 loss predisposes to hepatocellular-carcinoma by impairing liver regeneration and altering the liver lipid metabolic ground-state.
Germán Belenguer, Gianmarco Mastrogiovanni, Clare Pacini, Zoe Hall, Anna M Dowbaj, Robert Arnes-Benito, Aleksandra Sljukic, Nicole Prior, Sofia Kakava, Charles R Bradshaw, Susan Davies, Michele Vacca, Kourosh Saeb-Parsy, Bon-Kyoung Koo, Meritxell Huch
Feb 18, 2022
RNF43/ZNRF3 negatively regulate WNT signalling. Both genes are mutated in several types of cancers, however, their contribution to liver disease is unknown. Here we describe that hepatocyte-specific loss of Rnf43/Znrf3 results...
Adult Animals Carcinoma Hepatocellular Cell Differentiation Cell Proliferation Fatty Liver Gene Deletion Gene Expression Regulation Hepatocytes Hepatomegaly Humans Hyperplasia Lipid Droplets Lipid Metabolism lipidomics Liver Liver Neoplasms Liver Regeneration Mice Prognosis Ubiquitin-Protein Ligases
Liver organoids reproduce alpha-1 antitrypsin deficiency-related liver disease
Gema Gómez-Mariano, Nerea Matamala, Selene Martínez, Iago Justo, Alberto Marcacuzco, Carlos Jimenez, Sara Monzón, Isabel Cuesta, Cristina Garfia, María Teresa Martínez, Meritxell Huch, Ignacio Pérez de Castro, Manuel Posada, Sabina Janciauskiene, Beatriz Martínez-Delgado
Jun 06, 2020
Abstract: Background and aims: Alpha-1 antitrypsin (AAT) is a product of SERPINA1 gene mainly expressed by hepatocytes. Clinically relevant mutations in the SERPINA1 gene, such as Z (Glu342Lys), results in an expression of...
Defining Lineage Potential and Fate Behavior of Precursors during Pancreas Development.
Magdalena K Sznurkowska, Edouard Hannezo, Roberta Azzarelli, Steffen Rulands, Sonia Nestorowa, Christopher J Hindley, Jennifer Nichols, Berthold Göttgens, Meritxell Huch, Anna Philpott, Benjamin D Simons
Sep 20, 2018
Liver organoids reproduce alpha-1 antitrypsin deficiency-related liver disease.
Gema Gómez-Mariano, Nerea Matamala, Selene Martínez, Iago Justo, Alberto Marcacuzco, Carlos Jimenez, Sara Monzón, Isabel Cuesta, Cristina Garfia, María Teresa Martínez, Meritxell Huch, Ignacio Pérez de Castro, Manuel Posada, Sabina Janciauskiene, Beatriz Martínez-Delgado
Dec 12, 2020
BACKGROUND AND AIMS: Alpha-1 antitrypsin (AAT) is a product of SERPINA1 gene mainly expressed by hepatocytes. Clinically relevant mutations in the SERPINA1 gene, such as Z (Glu342Lys), results in an expression of misfolded AAT...
Over-expression of Plk4 induces centrosome amplification, loss of primary cilia and associated tissue hyperplasia in the mouse.
Paula A Coelho, Leah Bury, Marta N Shahbazi, Kifayathullah Liakath-Ali, Peri H Tate, Sam Wormald, Christopher J Hindley, Meritxell Huch, Joy Archer, William C Skarnes, Magdalena Zernicka-Goetz, David M Glover
Apr 15, 2019
To address the long-known relationship between supernumerary centrosomes and cancer, we have generated a transgenic mouse that permits inducible expression of the master regulator of centriole duplication, Polo-like-kinase-4...
Polo-like-kinase-4 centrosome amplification Pancreas primary cilia Skin tumour development Animals Cell Proliferation Cells Cultured Centrioles Centrosome Cilia Filaggrin Proteins Hyperplasia Intermediate Filament Proteins Islets of Langerhans Membrane Proteins Mice Protein Precursors Protein Serine-Threonine Kinases
Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids.
Nikitas Georgakopoulos, Nicole Prior, Brigitte Angres, Gianmarco Mastrogiovanni, Alex Cagan, Daisy Harrison, Christopher J Hindley, Robert Arnes-Benito, Siong-Seng Liau, Abbie Curd, Natasha Ivory, Benjamin D Simons, Inigo Martincorena, Helmut Wurst, Kourosh Saeb-Parsy, Meritxell Huch
Mar 28, 2020
BACKGROUND:Pancreatic organoid systems have recently been described for the in vitro culture of pancreatic ductal cells from mouse and human. Mouse pancreatic organoids exhibit unlimited expansion potential, while previously...
Published by: BMC developmental biology
Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids
Nikitas Georgakopoulos, Nicole Prior, Brigitte Angres, Gianmarco Mastrogiovanni, Alex Cagan, Daisy Harrison, Christopher J. Hindley, Robert Arnes-Benito, Siong-Seng Liau, Abbie Curd, Natasha Ivory, Benjamin D. Simons, Inigo Martincorena, Helmut Wurst, Kourosh Saeb-Parsy, Meritxell Huch
Feb 25, 2021
Abstract: Background: Pancreatic organoid systems have recently been described for the in vitro culture of pancreatic ductal cells from mouse and human. Mouse pancreatic organoids exhibit unlimited expansion potential, while...
Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis.
Lotta Hof, Till Moreth, Michael Koch, Tim Liebisch, Marina Kurtz, Julia Tarnick, Susanna M Lissek, Monique MA Verstegen, Luc JW van der Laan, Meritxell Huch, Franziska Matthäus, Ernst HK Stelzer, Francesco Pampaloni
Feb 24, 2021
Building consensus on definition and nomenclature of hepatic, pancreatic, and biliary organoids.
Ary Marsee, Floris JM Roos, Monique MA Verstegen, HPB Organoid Consortium, Helmuth Gehart, Eelco de Koning, Frédéric Lemaigre, Stuart J Forbes, Weng Chuan Peng, Meritxell Huch, Takanori Takebe, Ludovic Vallier, Hans Clevers, Luc JW van der Laan, Bart Spee
May 26, 2021
Hepatic, pancreatic, and biliary (HPB) organoids are powerful tools for studying development, disease, and regeneration. As organoid research expands, the need for clear definitions and nomenclature describing these systems also...
Metabolic Activation of Benzo[a]pyrene by Human Tissue Organoid Cultures.
Angela L Caipa Garcia, Jill E Kucab, Halh Al-Serori, Rebekah SS Beck, Franziska Fischer, Matthias Hufnagel, Andrea Hartwig, Andrew Floeder, Silvia Balbo, Hayley Francies, Mathew Garnett, Meritxell Huch, Jarno Drost, Matthias Zilbauer, Volker M Arlt, David H Phillips
Jan 24, 2023
Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids.
Nikitas Georgakopoulos, Nicole Prior, Brigitte Angres, Gianmarco Mastrogiovanni, Alex Cagan, Daisy Harrison, Christopher J Hindley, Robert Arnes-Benito, Siong-Seng Liau, Abbie Curd, Natasha Ivory, Benjamin D Simons, Inigo Martincorena, Helmut Wurst, Kourosh Saeb-Parsy, Meritxell Huch
Feb 05, 2020
BACKGROUND: Pancreatic organoid systems have recently been described for the in vitro culture of pancreatic ductal cells from mouse and human. Mouse pancreatic organoids exhibit unlimited expansion potential, while previously...
Chemically defined hydrogel Genetic stability In vivo safety Organoid Pancreas Primary cultures Cell Differentiation Cells Cultured Female Flow Cytometry Genomic Instability Humans In Vitro Techniques Lentivirus Male Organ Culture Techniques Organoids Pancreas Reverse Transcriptase Polymerase Chain Reaction
Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids
Nikitas Georgakopoulos, Nicole Prior, Brigitte Angres, Gianmarco Mastrogiovanni, Alex Cagan, Daisy Harrison, Christopher J. Hindley, Robert Arnes-Benito, Siong-Seng Liau, Abbie Curd, Natasha Ivory, Benjamin D. Simons, Inigo Martincorena, Helmut Wurst, Kourosh Saeb-Parsy, Meritxell Huch
Jun 06, 2020
Abstract: Background: Pancreatic organoid systems have recently been described for the in vitro culture of pancreatic ductal cells from mouse and human. Mouse pancreatic organoids exhibit unlimited expansion potential, while...
Metabolic Activation of Benzo[a]pyrene by Human Tissue Organoid Cultures.
Angela L Caipa Garcia, Jill E Kucab, Halh Al-Serori, Rebekah SS Beck, Franziska Fischer, Matthias Hufnagel, Andrea Hartwig, Andrew Floeder, Silvia Balbo, Hayley Francies, Mathew Garnett, Meritxell Huch, Jarno Drost, Matthias Zilbauer, Volker M Arlt, David H Phillips
Feb 09, 2023
Organoids are 3D cultures that to some extent reproduce the structure, composition and function of the mammalian tissues from which they derive, thereby creating in vitro systems with more in vivo-like characteristics than 2D...
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