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Results: 301
Codon usage optimization in pluripotent embryonic stem cells
Abstract: Background: The uneven use of synonymous codons in the transcriptome regulates the efficiency and fidelity of protein translation rates. Yet, the importance of this codon bias in regulating cell state-specific...
Specification and epigenetic programming of the human germ line.
Primordial germ cells (PGCs), the precursors of sperm and eggs, are established in perigastrulation-stage embryos in mammals. Signals from extra-embryonic tissues induce a unique gene regulatory network in germline-competent...
Disabling de novo DNA methylation in embryonic stem cells allows an illegitimate fate trajectory.
Masaki Kinoshita, Meng Amy Li, Michael Barber, William Mansfield, Sabine Dietmann, Austin Smith
Sep 01, 2021
Genome remethylation is essential for mammalian development but specific reasons are unclear. Here we examined embryonic stem (ES) cell fate in the absence of de novo DNA methyltransferases. We observed that ES cells deficient...
Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development.
Jan J Zylicz, Sabine Dietmann, Ufuk Günesdogan, Jamie A Hackett, Delphine Cougot, Caroline Lee, M Azim Surani
Nov 24, 2015
Chromosomes developmental biology Epiblast epigenetics Genes histone MOUSE post-implantation primed pluripotency Stem Cells Animals Chromatin Embryonic Development Enhancer of Zeste Homolog 2 Protein Gene Expression Regulation Developmental Histone-Lysine N-Methyltransferase Histones Methylation Mice Polycomb Repressive Complex 2 Protein Processing Post-Translational
RN7SK small nuclear RNA controls bidirectional transcription of highly expressed gene pairs in skin
Roberto Bandiera, Rebecca E. Wagner, Thiago Britto-Borges, Christoph Dieterich, Sabine Dietmann, Susanne Bornelöv, Michaela Frye
Oct 07, 2021
Abstract: Pausing of RNA polymerase II (Pol II) close to promoters is a common regulatory step in RNA synthesis, and is coordinated by a ribonucleoprotein complex scaffolded by the noncoding RNA RN7SK. The function of...
Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency.
Masaki Kinoshita, Michael Barber, William Mansfield, Yingzhi Cui, Daniel Spindlow, Giuliano Giuseppe Stirparo, Sabine Dietmann, Jennifer Nichols, Austin Smith
Nov 12, 2020
G9a regulates temporal preimplantation developmental program and lineage segregation in blastocyst
Jan J Zylicz, Maud Borensztein, Frederick CK Wong, Yun Huang, Caroline Lee, Sabine Dietmann, Azim Surani
Sep 28, 2018
A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons
Anastasiya Sybirna, Walfred W. C. Tang, Merrick Pierson Smela, Sabine Dietmann, Wolfram H. Gruhn, Ran Brosh, M. Azim Surani
Mar 09, 2021
Exit from Naive Pluripotency Induces a Transient X Chromosome Inactivation-like State in Males.
Elsa J Sousa, Hannah T Stuart, Lawrence E Bates, Mohammadmersad Ghorbani, Jennifer Nichols, Sabine Dietmann, José CR Silva
Aug 03, 2018
RN7SK small nuclear RNA controls bidirectional transcription of highly expressed gene pairs in skin.
Roberto Bandiera, Rebecca E Wagner, Thiago Britto-Borges, Christoph Dieterich, Sabine Dietmann, Susanne Bornelöv, Michaela Frye
Nov 08, 2021
Animals Cell Cycle Cell Differentiation Cell Proliferation Chromatin Chromatin Assembly and Disassembly epidermis Female Gene Expression Regulation Humans Keratinocytes Mice Mice Inbred C57BL Mice Knockout Promoter Regions Genetic RNA Polymerase II RNA Splicing RNA Small Nuclear Skin Stem Cells Transcription Genetic
SOX17 is a critical specifier of human primordial germ cell fate.
Naoko Irie, Leehee Weinberger, Walfred WC Tang, Toshihiro Kobayashi, Sergey Viukov, Yair S Manor, Sabine Dietmann, Jacob H Hanna, M Azim Surani
Mar 20, 2015
NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers.
Kazuhiro Murakami, Ufuk Günesdogan, Jan J Zylicz, Walfred WC Tang, Roopsha Sengupta, Toshihiro Kobayashi, Shinseog Kim, Richard Butler, Sabine Dietmann, M Azim Surani
Dec 09, 2015
Activins Animals Bone Morphogenetic Protein 4 Cell Differentiation Chromatin DNA-Binding Proteins Enhancer Elements Genetic Epigenesis Genetic Female Fibroblast Growth Factor 2 Gene Expression Regulation Developmental Genome Germ Cells Germ Layers Homeodomain Proteins Male Mice Mouse embryonic stem cells Nanog Homeobox Protein Pluripotent Stem Cells Positive Regulatory Domain I-Binding Factor 1 Protein Binding RNA-Binding Proteins SOXB1 Transcription Factors Transcription Factor AP-2 Transcription Factors
Loss of 5-methylcytosine alters the biogenesis of vault-derived small RNAs to coordinate epidermal differentiation.
Abdulrahim A Sajini, Nila Roy Choudhury, Rebecca E Wagner, Susanne Bornelöv, Tommaso Selmi, Christos Spanos, Sabine Dietmann, Juri Rappsilber, Gracjan Michlewski, Michaela Frye
Apr 18, 2019
A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons.
Anastasiya Sybirna, Walfred W C Tang, Merrick Pierson Smela, Sabine Dietmann, Wolfram H Gruhn, Ran Brosh, M Azim Surani
Apr 11, 2020
PRDM14 is a crucial regulator of mouse primordial germ cells (mPGCs), epigenetic reprogramming and pluripotency, but its role in the evolutionarily divergent regulatory network of human PGCs (hPGCs) remains unclear. Besides, a...
Published by: Nature Communications
A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons.
Anastasiya Sybirna, Walfred WC Tang, Merrick Pierson Smela, Sabine Dietmann, Wolfram H Gruhn, Ran Brosh, M Azim Surani
Jan 15, 2020
Functional Studies of Missense TREM2 Mutations in Human Stem Cell-Derived Microglia.
Philip W Brownjohn, James Smith, Ravi Solanki, Ebba Lohmann, Henry Houlden, John Hardy, Sabine Dietmann, Frederick J Livesey
Jun 04, 2018
The derivation of microglia from human stem cells provides systems for understanding microglial biology and enables functional studies of disease-causing mutations. We describe a robust method for the derivation of human...
Single cell transcriptome analysis of human, marmoset and mouse embryos reveals common and divergent features of preimplantation development.
Thorsten Boroviak, Giuliano G Stirparo, Sabine Dietmann, Irene Hernando-Herraez, Hisham Mohammed, Wolf Reik, Austin Smith, Erika Sasaki, Jennifer Nichols, Paul Bertone
Dec 06, 2018
Blastocyst Embryo human Inner cell mass Pluripotency primate Animals Callithrix Conserved Sequence Embryo Mammalian Embryonic Development Endoderm Epigenesis Genetic Gene Expression Profiling Gene Expression Regulation Developmental Gene Regulatory Networks Germ Layers Humans Mice Otx Transcription Factors RNA Messenger Ribosomes Single-Cell Analysis Transcription Genetic Transcriptome
The Nucleosome Remodelling and Deacetylation complex suppresses transcriptional noise during lineage commitment.
Thomas Burgold, Michael Barber, Susan Kloet, Julie Cramard, Sarah Gharbi, Robin Floyd, Masaki Kinoshita, Meryem Ralser, Michiel Vermeulen, Nicola Reynolds, Sabine Dietmann, Brian Hendrich
Apr 12, 2019
ES cell NuRD Chromatin Lineage commitment Transcription Animals Cell Differentiation Cell Lineage Cells Cultured Cellular Reprogramming DNA-Binding Proteins Embryo Mammalian Gene Expression Regulation Developmental Mi-2 Nucleosome Remodeling and Deacetylase Complex Mice Mice Knockout Mouse embryonic stem cells Neoplasm Proteins Repressor Proteins Signal-To-Noise Ratio Trans-Activators Transcription Factors Transcription Genetic
Epigenetic resetting in the human germ line entails histone modification remodeling.
Wolfram H Gruhn, Walfred WC Tang, Sabine Dietmann, João P Alves-Lopes, Christopher A Penfold, Frederick CK Wong, Navin B Ramakrishna, M Azim Surani
Feb 19, 2023
Epigenetic resetting in the mammalian germ line entails acute DNA demethylation, which lays the foundation for gametogenesis, totipotency, and embryonic development. We characterize the epigenome of hypomethylated human...
Derivation of hypermethylated pluripotent embryonic stem cells with high potency.
Siqin Bao, Walfred WC Tang, Baojiang Wu, Shinseog Kim, Jingyun Li, Lin Li, Toshihiro Kobayashi, Caroline Lee, Yanglin Chen, Mengyi Wei, Shudong Li, Sabine Dietmann, Fuchou Tang, Xihe Li, M Azim Surani
Feb 27, 2018
Animals Basic Helix-Loop-Helix Transcription Factors Chimera DNA Methylation Epidermal Growth Factor Germ Layers Homeodomain Proteins Membrane Glycoproteins Mice Mice Inbred C57BL Mouse embryonic stem cells Neoplasm Proteins Oncogene Protein p21(ras) Sequence Analysis RNA T-Box Domain Proteins Wnt-5a Protein
The Nucleosome Remodeling and Deacetylation Complex Modulates Chromatin Structure at Sites of Active Transcription to Fine-Tune Gene Expression.
Susanne Bornelöv, Nicola Reynolds, Maria Xenophontos, Sarah Gharbi, Ewan Johnstone, Robin Floyd, Meryem Ralser, Jason Signolet, Remco Loos, Sabine Dietmann, Paul Bertone, Brian Hendrich
Oct 10, 2018
Mediator NuRD RNA Polymerase II Chromatin Embryonic Stem Cells ENHANCER Transcription Transcription factor Acetylation Animals Cell Line Gene Expression Regulation Histones Mi-2 Nucleosome Remodeling and Deacetylase Complex Mice Mouse embryonic stem cells Nucleosomes RNA Polymerase II Transcription Initiation Site Transcription Genetic
A Unique Gene Regulatory Network Resets the Human Germline Epigenome for Development.
Walfred WC Tang, Sabine Dietmann, Naoko Irie, Harry G Leitch, Vasileios I Floros, Charles R Bradshaw, Jamie A Hackett, Patrick F Chinnery, M Azim Surani
Jun 04, 2015
PRMT5 protects genomic integrity during global DNA demethylation in primordial germ cells and preimplantation embryos.
Shinseog Kim, Ufuk Günesdogan, Jan J Zylicz, Jamie A Hackett, Delphine Cougot, Siqin Bao, Caroline Lee, Sabine Dietmann, George E Allen, Roopsha Sengupta, M Azim Surani
Apr 14, 2015
Animals Apoptosis Blastocyst Cells Cultured DNA Damage DNA Methylation DNA Transposable Elements Embryonic Development Embryonic Stem Cells Female Genomic Instability Histones Male Mice Transgenic Ovum Protein Methyltransferases Protein Processing Post-Translational Protein-Arginine N-Methyltransferases Spermatozoa
Specification and epigenomic resetting of the pig germline exhibit conservation with the human lineage.
Qifan Zhu, Fei Sang, Sarah Withey, Walfred Tang, Sabine Dietmann, Doris Klisch, Priscila Ramos-Ibeas, Haixin Zhang, Cristina E Requena, Petra Hajkova, Matt Loose, M Azim Surani, Ramiro Alberio
Feb 18, 2021
Investigations of the human germline and programming are challenging because of limited access to embryonic material. However, the pig as a model may provide insights into transcriptional network and epigenetic reprogramming...
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