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Results: 584
Superresolving the kidney-a practical comparison of fluorescence nanoscopy of the glomerular filtration barrier.
Lucia Cs Wunderlich, Florian Ströhl, Stefan Ströhl, Oliver Vanderpoorten, Luca Mascheroni, Clemens F Kaminski
Feb 12, 2021
Immunofluorescence microscopy is routinely used in the diagnosis of and research on renal impairments. However, this highly specific technique is restricted in its maximum resolution to about 250 nm in the lateral and 700 nm in...
Fluorescence microscopy formalin-fixed paraffin-embedded Kidney Nephrin Podocyte foot processes super-resolution microscopy Animals Glomerular Filtration Barrier Humans Kidney Membrane Proteins Mice Inbred C57BL Microscopy Fluorescence Paraffin Embedding Podocytes single molecule imaging Tissue Fixation
A concept for single-shot volumetric fluorescence imaging via orthogonally polarized excitation lattices.
The deconvolution of widefield fluorescence images provides only guesses of spatial frequency information along the optical axis due to the so called missing cone in the optical transfer function. Retaining the single-shot...
Flat-Field Super-Resolution Localization Microscopy with a Low-Cost Refractive Beam-Shaping Element.
Christopher J Rowlands, Florian Ströhl, Pedro P Vallejo Ramirez, Katharina M Scherer, Clemens F Kaminski
Sep 05, 2018
Super-resolution single-molecule localization microscopy, often referred to as PALM/STORM, works by ensuring that fewer than one fluorophore in a diffraction-limited volume is emitting at any one time, allowing the observer to...
A waveguide imaging platform for live-cell TIRF imaging of neurons over large fields of view.
Ida S Opstad, Florian Ströhl, Marcus Fantham, Colin Hockings, Oliver Vanderpoorten, Francesca W van Tartwijk, Julie Qiaojin Lin, Jean-Claude Tinguely, Firehun T Dullo, Gabriele S Kaminski-Schierle, Balpreet S Ahluwalia, Clemens F Kaminski
Apr 14, 2020
Large fields of view (FOVs) in total internal reflection fluorescence microscopy (TIRFM) via waveguides have been shown to be highly beneficial for single molecule localisation microscopy on fixed cells [1,2] and have also been...
Super-condenser enables labelfree nanoscopy.
Florian Ströhl, Ida S Opstad, Jean-Claude Tinguely, Firehun T Dullo, Ioanna Mela, Johannes WM Osterrieth, Balpreet S Ahluwalia, Clemens F Kaminski
Jul 24, 2019
Labelfree nanoscopy encompasses optical imaging with resolution in the 100 nm range using visible wavelengths. Here, we present a labelfree nanoscopy method that combines coherent imaging techniques with waveguide microscopy to...
On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons.
Toshiaki Shigeoka, Max Koppers, Hovy Ho-Wai Wong, Julie Qiaojin Lin, Roberta Cagnetta, Asha Dwivedy, Janaina de Freitas Nascimento, Francesca W van Tartwijk, Florian Ströhl, Jean-Michel Cioni, Julia Schaeffer, Mark Carrington, Clemens F Kaminski, Hosung Jung, William A Harris, Christine E Holt
Nov 21, 2019
The Edgeworth Cube
Social peace is an asset to every society. Its absence endangers the well-being and the safety of the population and the stability of states. In order to better understand the interdependencies of poverty, social peace and...
Published by: IGI Global Scientific Publishing
How Can Accessibility for Deaf and Hearing-Impaired Players be Improved in Video Games?
This research investigates how the accessibility of video games can be improved for deaf and hearing-impaired players. The journal is divided into several areas, first, examining the use of subtitles and closed captions used in...
Published by: IGI Global Scientific Publishing
Creating a Functional Interdependency Map for Supporting the “Act of Improvement” in Business Process Improvement Projects
Business process improvement (BPI) is of high priority for practitioners. But especially the most value-adding phase in a BPI project, namely the “act of improvement”, is insufficiently supported despite the many existing...
Published by: IGI Global Scientific Publishing
OptiJ
Pedro P Vallejo Ramirez, Joseph Zammit, Oliver Vanderpoorten, Fergus Riche, Francois-Xavier Blé, Xiao-Hong Zhou, Bogdan Spiridon, Christopher Valentine, Simeon E Spasov, Pelumi W Oluwasanya, Gemma Goodfellow, Marcus J Fantham, Omid Siddiqui, Farah Alimagham, Miranda Robbins, Andrew Stretton, Dimitrios Simatos, Oliver Hadeler, Eric J Rees, Florian Ströhl, Romain F Laine, Clemens F Kaminski
Oct 10, 2019
The three-dimensional imaging of mesoscopic samples with Optical Projection Tomography (OPT) has become a powerful tool for biomedical phenotyping studies. OPT uses visible light to visualize the 3D morphology of large...
FUS Phase Separation Is Modulated by a Molecular Chaperone and Methylation of Arginine Cation-π Interactions.
Seema Qamar, GuoZhen Wang, Suzanne J Randle, Francesco Simone Ruggeri, Juan A Varela, Julie Qiaojin Lin, Emma C Phillips, Akinori Miyashita, Declan Williams, Florian Ströhl, William Meadows, Rodylyn Ferry, Victoria J Dardov, Gian G Tartaglia, Lindsay A Farrer, Gabriele S Kaminski Schierle, Clemens F Kaminski, Christine E Holt, Paul E Fraser, Gerold Schmitt-Ulms, David Klenerman, Tuomas Knowles, Michele Vendruscolo, Peter St George-Hyslop
Sep 05, 2018
Reversible phase separation underpins the role of FUS in ribonucleoprotein granules and other membrane-free organelles and is, in part, driven by the intrinsically disordered low-complexity (LC) domain of FUS. Here, we report...
AFM-IR arginine methylation cation-π citrullination frontotemporal dementia membraneless organelle neuronal ribonucleoprotein granule phase separation phase-sensitive fluorescent dyes synaptic new protein synthesis Amyotrophic Lateral Sclerosis Animals Arginine cations DNA Methylation frontotemporal dementia Frontotemporal Lobar Degeneration Humans Microscopy Atomic Force Microscopy Fluorescence Molecular Chaperones Protein Binding Protein Domains Protein Processing Post-Translational Protein Structure Secondary RNA-Binding Protein FUS Tyrosine Xenopus laevis
OptiJ
Pedro P. Vallejo Ramirez, Joseph Zammit, Oliver Vanderpoorten, Fergus Riche, Francois-Xavier Blé, Xiao-Hong Zhou, Bogdan Spiridon, Christopher Valentine, Simeon E. Spasov, Pelumi W. Oluwasanya, Gemma Goodfellow, Marcus J. Fantham, Omid Siddiqui, Farah Alimagham, Miranda Robbins, Andrew Stretton, Dimitrios Simatos, Oliver Hadeler, Eric J. Rees, Florian Ströhl, Romain F. Laine, Clemens F. Kaminski
Oct 29, 2020
Abstract: The three-dimensional imaging of mesoscopic samples with Optical Projection Tomography (OPT) has become a powerful tool for biomedical phenotyping studies. OPT uses visible light to visualize the 3D morphology of large...
C-terminal calcium binding of α-synuclein modulates synaptic vesicle interaction.
Janin Lautenschläger, Amberley D Stephens, Giuliana Fusco, Florian Ströhl, Nathan Curry, Maria Zacharopoulou, Claire H Michel, Romain Laine, Nadezhda Nespovitaya, Marcus Fantham, Dorothea Pinotsi, Wagner Zago, Paul Fraser, Anurag Tandon, Peter St George-Hyslop, Eric Rees, Jonathan J Phillips, Alfonso De Simone, Clemens F Kaminski, Gabriele S Kaminski Schierle
Jul 31, 2018
Alpha-synuclein is known to bind to small unilamellar vesicles (SUVs) via its N terminus, which forms an amphipathic alpha-helix upon membrane interaction. Here we show that calcium binds to the C terminus of alpha-synuclein...
Iterative and Semi-Supervised Design of Chatbots Using Interactive Clustering
Chatbots represent a promising tool to automate the processing of requests in a business context. However, despite major progress in natural language processing technologies, constructing a dataset deemed relevant by business...
Published by: IGI Global Scientific Publishing
Digitally Supported Participation Processes and Tools to Promote Gender-Responsive Public Spaces
Flora Fessler, Florian Reinwald, Roswitha Weichselbaumer, Ernst Gebetsroither-Geringer, Jana Wentz
Jan 01, 2024
Planning is faced with the challenge of designing public open spaces that meet the diverse needs of everyday life. Gender-sensitive planning can facilitate the development of gender-responsive public spaces by considering the...
Published by: IGI Global Scientific Publishing
The Effects of Asset Allocation and Active Management on Total Return of Managed Funds
Florian Halili
Jan 01, 0001
Portfolio managers are charged with maximizing returns for a given level of risk. There are practical problems that arise in creating an efficient portfolio and maintaining a target level of risk and return. This paper will...
portfolio policy Asset Allocation risk active management investment rebalancing security selection Business Economics Finance and Financial Management Portfolio and Security Analysis Social and Behavioral Sciences Strategic Management Policy
Published by: Ursinus College
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