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A NuRD Complex from Xenopus laevis Eggs Is Essential for DNA Replication during Early Embryogenesis.
Christo P Christov, Kevin S Dingwell, Mark Skehel, Helen S Wilkes, Julian E Sale, James C Smith, Torsten Krude
Apr 20, 2018
DNA replication in the embryo of Xenopus laevis changes dramatically at the mid-blastula transition (MBT), with Y RNA-independent random initiation switching to Y RNA-dependent initiation at specific origins. Here, we identify...
DNA Replication NuRD Xenopus laevis Y RNA chromatin remodeling development Histone deacetylase initiation mid-blastula transition Non-coding RNA Animals Blastula Cell Extracts DNA Replication Embryonic Development HeLa Cells Humans Mi-2 Nucleosome Remodeling and Deacetylase Complex Ovum RNA Xenopus laevis
Controlled Ligand Exchange Between Ruthenium Organometallic Cofactor Precursors and a Naïve Protein Scaffold Generates Artificial Metalloenzymes Catalysing Transfer Hydrogenation
George S Biggs, Oskar James Klein, Sarah L Maslen, J Mark Skehel, Trevor J Rutherford, Stefan MV Freund, Florian Hollfelder, Sally R Boss, Paul D Barker
Apr 26, 2021
Crystal structure of the N-terminal domain of human Timeless and its interaction with Tipin
S Holzer, G Degliesposti, ML Kilkenny, SL Maslen, D Matak-Vinkovíc, M Skehel, L Pellegrini
Apr 11, 2017
Biophysical Phenomena Carrier Proteins Cell Cycle Proteins Cross-Linking Reagents Crystallography X-Ray DNA-Binding Proteins Humans Intracellular Signaling Peptides and Proteins Mass Spectrometry Models Molecular Nuclear Proteins Protein Binding Protein Domains Protein Multimerization Structural Homology Protein
Controlled Ligand Exchange Between Ruthenium Organometallic Cofactor Precursors and a Naïve Protein Scaffold Generates Artificial Metalloenzymes Catalysing Transfer Hydrogenation
George S. Biggs, Oskar James Klein, Sarah L. Maslen, J. Mark Skehel, Trevor J. Rutherford, Stefan M. V. Freund, Florian Hollfelder, Sally R. Boss, Paul D. Barker
Apr 26, 2021
RESEARCH ARTICLE RESEARCH ARTICLES direct coordination Ligand exchange metalloenzymes Ruthenium transfer hydrogenation
Published by: Angewandte Chemie International Edition
Controlled Ligand Exchange Between Ruthenium Organometallic Cofactor Precursors and a Naïve Protein Scaffold Generates Artificial Metalloenzymes Catalysing Transfer Hydrogenation.
George S Biggs, Oskar James Klein, Sarah L Maslen, J Mark Skehel, Trevor J Rutherford, Stefan MV Freund, Florian Hollfelder, Sally R Boss, Paul D Barker
Mar 09, 2021
Many natural metalloenzymes assemble from proteins and biosynthesised complexes, generating potent catalysts by changing metal coordination. Here we adopt the same strategy to generate artificial metalloenzymes (ArMs) using...
Phosphorylation-dependent BRD4 dimerization and implications for therapeutic inhibition of BET family proteins.
Francesca Malvezzi, Christopher J Stubbs, Thomas A Jowitt, Ian L Dale, Xieyang Guo, Jon P DeGnore, Gianluca Degliesposti, J Mark Skehel, Andrew J Bannister, Mark S McAlister
Jan 07, 2022
Bipartite binding and partial inhibition links DEPTOR and mTOR in a mutually antagonistic embrace.
Maren Heimhalt, Alex Berndt, Jane Wagstaff, Madhanagopal Anandapadamanaban, Olga Perisic, Sarah Maslen, Stephen McLaughlin, Conny Wing-Heng Yu, Glenn R Masson, Andreas Boland, Xiaodan Ni, Keitaro Yamashita, Garib N Murshudov, Mark Skehel, Stefan M Freund, Roger L Williams
Sep 14, 2021
The mTORC1 kinase complex regulates cell growth, proliferation, and survival. Because mis-regulation of DEPTOR, an endogenous mTORC1 inhibitor, is associated with some cancers, we reconstituted mTORC1 with DEPTOR to understand...
DEPTOR Biochemistry Cancer chemical biology cryo-EM human mTOR molecular biophysics partial inhibition Signal Transduction structural biology Binding Sites Cryoelectron Microscopy Escherichia coli Gene Expression Regulation Humans Image Processing Computer-Assisted Intracellular Signaling Peptides and Proteins Mechanistic Target of Rapamycin Complex 1 Models Molecular PDZ Domains Protein Binding Protein Conformation Recombinant Proteins TOR Serine-Threonine Kinases
The earth's electromagnetic environment
It is found that the energy density of electromagnetic fields at the surface of the Earth follow a scaling law that extends over 16 orders of magnitude from 10-9 Hz to 107 Hz. The temporal variability of the field can be...
The earth's electromagnetic environment
It is found that the energy density of electromagnetic fields at the surface of the Earth follow a scaling law that extends over 16 orders of magnitude from 10-9 Hz to 107 Hz. The temporal variability of the field can be...
Profiling the Site of Protein CoAlation and Coenzyme A Stabilization Interactions.
Maria-Armineh Tossounian, Maria Baczynska, William Dalton, Charlie Newell, Yilin Ma, Sayoni Das, Jonathan Alexis Semelak, Dario Ariel Estrin, Valeriy Filonenko, Madia Trujillo, Sew Yeu Peak-Chew, Mark Skehel, Franca Fraternali, Christine Orengo, Ivan Gout
Jul 18, 2022
Coenzyme A (CoA) is a key cellular metabolite known for its diverse functions in metabolism and regulation of gene expression. CoA was recently shown to play an important antioxidant role under various cellular stress conditions...
Architecture of the biofilm-associated archaic Chaperone-Usher pilus CupE from Pseudomonas aeruginosa.
Jan Böhning, Adrian W Dobbelstein, Nina Sulkowski, Kira Eilers, Andriko von Kügelgen, Abul K Tarafder, Sew-Yeu Peak-Chew, Mark Skehel, Vikram Alva, Alain Filloux, Tanmay AM Bharat
Apr 14, 2023
Dynamic exergy analysis
As the power and transport sectors decarbonize, industrial emissions will become the main focus of decarbonization efforts. Exergy analysis provides a combined material and energy efficiency approach to assess industrial plants...
Redox Regulation of the Quorum-sensing Transcription Factor AgrA by Coenzyme A.
Jovana Baković, Bess Yi Kun Yu, Daniel Silva, Maria Baczynska, Sew Yeu Peak-Chew, Amy Switzer, Lynn Burchell, Sivaramesh Wigneshweraraj, Muralidharan Vandanashree, Balasubramanian Gopal, Valeriy Filonenko, Mark Skehel, Ivan Gout
May 26, 2021
Staphylococcus aureus (S. aureus) is an aggressive opportunistic pathogen of prominent virulence and antibiotic resistance. These characteristics are due in part to the accessory gene regulator (agr) quorum-sensing system, which...
Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes.
Shirley Tremel, Yohei Ohashi, Dustin R Morado, Jessie Bertram, Olga Perisic, Laura TL Brandt, Marie-Kristin von Wrisberg, Zhuo A Chen, Sarah L Maslen, Oleksiy Kovtun, Mark Skehel, Juri Rappsilber, Kathrin Lang, Sean Munro, John AG Briggs, Roger L Williams
Mar 10, 2021
The lipid phosphatidylinositol-3-phosphate (PI3P) is a regulator of two fundamental but distinct cellular processes, endocytosis and autophagy, so its generation needs to be under precise temporal and spatial control. PI3P is...
Partisan Gerrymandering and the Illusion of Unfairness
JM Eisler
Aug 22, 2017
Partisan gerrymandering is frequently condemned for distorting democracy and causing unfair representation, and many reformers have called upon federal courts to prohibit the practice. However, the judiciary has struggled to...
Production, consumers' convenience, and cynical economies
JM del Nido
May 06, 2021
Based on twelve months of fieldwork into Uber's conflict in Buenos Aires, Argentina, this article examines convenience's role in the emergence of what I call cynical economies: a method and logic of production expressly...
Fibre-reinforced polymers in reinforced and prestressed concrete applications
JM Lees
Sep 10, 2013
Recent developments in the use of fibre-reinforced polymers (FRPs) in reinforced and prestressed concrete applications are reviewed. The influence of FRP material properties on the design of concrete structures is considered and...
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