Search
Results: 234
Formation of hippocampal mHTT aggregates leads to impaired spatial memory, hippocampal activation and adult neurogenesis.
Adult neurogenesis Dentate Gyrus Huntingtin Lentiviral vectors Memory Neural progenitor cells Animals Disease Models Animal Female Genetic Vectors Hippocampus Humans Huntingtin Protein Huntington Disease Lentivirus Maze Learning Memory Disorders Mice Inbred C57BL Mutation Neurogenesis Neurons protein aggregation Pathological Proto-Oncogene Proteins c-fos Spatial Memory
Organoid Sample Preparation and Extraction for LC-MS Peptidomics.
Emily L Miedzybrodzka, Rachel E Foreman, Sam G Galvin, Pierre Larraufie, Amy L George, Deborah A Goldspink, Frank Reimann, Fiona M Gribble, Richard G Kay
Mar 18, 2021
This protocol describes the peptidomic analysis of organoid lysates, FACS-purified cell populations, and 2D culture secretions by liquid chromatography mass spectrometry (LC-MS). Currently, most peptides are quantified by ELISA...
LC–MS Lipidomics
Typical lipidomics methods incorporate a liquid−liquid extraction with LC−MS quantitation; however, the classic sample extraction methods are not high-throughput and do not perform well at extracting the full range of lipids...
Measurement of the total angiotensinogen and its reduced and oxidised forms in human plasma using targeted LC-MS/MS.
Lina A Dahabiyeh, David Tooth, Robin W Carrell, Randy J Read, Yahui Yan, Fiona Broughton Pipkin, David A Barrett
Dec 11, 2018
Angiotensinogen (AGT) is a critical protein in the renin-angiotensin-aldosterone system and may have an important role in the pathogenesis of pre-eclampsia. The disulphide linkage between cysteines 18 and 138 has a key role in...
Functionalized graphene oxide tablets for sample preparation of drugs in biological fluids
Zeynab Zohdijamil, Mahdi Hashemi, Abbi Abdel-Rehim, Karthik Laxman, Abdusalam Uheida, Joydeep Dutta, Mohamed Abdel-Rehim
Jun 04, 2021
In this work, graphene oxide-based tablets (GO-Tabs) were prepared by applying a thin layer of functionalized GO on a polyethylene substrate. The GO was functionalized with amine groups (-NH2 ) by poly(ethylene...
Isolation, identification and quantitation of hydroxycinnamic acid conjugates, potential platform chemicals, in the leaves and stems of Miscanthus x giganteus using LC-ESI-MS(n)
Miscanthus x giganteus is a source of platform chemicals and bioethanol through fermentation. Cinnamates in leaves and stems were analysed by LC-ESI-MS(n). Free phenols were extracted and separated chromatographically. More than...
Isolation, identification and quantitation of hydroxycinnamic acid conjugates, potential platform chemicals, in the leaves and stems of Miscanthus x giganteus using LC-ESI-MS(n)
Miscanthus x giganteus is a source of platform chemicals and bioethanol through fermentation. Cinnamates in leaves and stems were analysed by LC-ESI-MS(n). Free phenols were extracted and separated chromatographically. More than...
A novel method for quantification of gemcitabine and its metabolites 2',2'-difluorodeoxyuridine and gemcitabine triphosphate in tumour tissue by LC-MS/MS
Tashinga E Bapiro, Frances M Richards, Mae A Goldgraben, Kenneth P Olive, Basetti Madhu, Kristopher K Frese, Natalie Cook, Michael A Jacobetz, Donna-Michelle Smith, David A Tuveson, John R Griffiths, Duncan I Jodrell
Nov 30, 2017
PURPOSE: To develop a sensitive analytical method to quantify gemcitabine (2',2'-difluorodeoxycytidine, dFdC) and its metabolites 2',2'-difluorodeoxyuridine (dFdU) and 2',2'-difluorodeoxycytidine-5'-triphosphate (dFdCTP)...
A comparison of murine epidermal Langerhans cells with spleen dendritic cells
To establish if epidermal Langerhans cells (LC) are related to spleen dendritic cells, we have considered the morphology, phenotype, and function of the 2 cell types in culture. Cultured LC could be partially enriched (up to...
Published by: Rockefeller University
Development and validation of an LC-MS/MS method for detection and quantification of in vivo derived metabolites of [Pyr1]apelin-13 in humans.
Duuamene Nyimanu, Richard G Kay, Petra Sulentic, Rhoda E Kuc, Philip Ambery, Lutz Jermutus, Frank Reimann, Fiona M Gribble, Joseph Cheriyan, Janet J Maguire, Anthony P Davenport
Dec 05, 2019
[Pyr1]apelin-13 is the predominant apelin peptide isoform in the human cardiovascular system and plasma. To date, few studies have investigated [Pyr1]apelin-13 metabolism in vivo in rats with no studies examining its stability...
Development and validation of an LC-MS/MS method for detection and quantification of in vivo derived metabolites of [Pyr 1 ]apelin-13 in humans
Duuamene Nyimanu, Richard G. Kay, Petra Sulentic, Rhoda E. Kuc, Philip Ambery, Lutz Jermutus, Frank Reimann, Fiona M. Gribble, Joseph Cheriyan, Janet J. Maguire, Anthony P. Davenport
Dec 26, 2020
Abstract: [Pyr1]apelin-13 is the predominant apelin peptide isoform in the human cardiovascular system and plasma. To date, few studies have investigated [Pyr1]apelin-13 metabolism in vivo in rats with no studies examining its...
Development and validation of an LC-MS/MS method for detection and quantification of in vivo derived metabolites of [Pyr1]apelin-13 in humans.
Duuamene Nyimanu, Richard G Kay, Petra Sulentic, Rhoda E Kuc, Philip Ambery, Lutz Jermutus, Frank Reimann, Fiona M Gribble, Joseph Cheriyan, Janet J Maguire, Anthony P Davenport
Jan 29, 2020
[Pyr1]apelin-13 is the predominant apelin peptide isoform in the human cardiovascular system and plasma. To date, few studies have investigated [Pyr1]apelin-13 metabolism in vivo in rats with no studies examining its stability...
Noradrenergic-dependent functions are associated with age-related locus coeruleus signal intensity differences.
Kathy Y Liu, Rogier A Kievit, Kamen A Tsvetanov, Matthew J Betts, Emrah Düzel, James B Rowe, Robert Howard, Dorothea Hämmerer
May 06, 2020
The locus coeruleus (LC), the origin of noradrenergic modulation of cognitive and behavioral function, may play an important role healthy ageing and in neurodegenerative conditions. We investigated the functional significance of...
Published by: Nature Communications
Lateral compression type 1 fracture fixation in the elderly (L1FE)
Elizabeth Cook, Joanne Laycock, Mehool Acharya, Michael Ross Backhouse, Belen Corbacho, Laura Doherty, Daren Forward, Catherine Hewitt, Catherine Hilton, Peter Hull, Jamila Kassam, Camila Maturana, Catriona Mcdaid, Jenny Roche, Dhanupriya Sivapathasuntharam, David Torgerson, Peter Bates
Mar 05, 2023
BACKGROUND: Lateral compression type1 (LC-1) fragility fractures are a common, painful injury in older adults resulting in reduced mobility. The incidence of these fractures is increasing with the growing older adult population....
Elderly patients fragility fracture INFIX surgery Immobility-related complications LC-1 Lateral compression type 1 older adults Osteoporotic bone Pelvic fracture fixation Pubic ramus fracture Aged Humans Middle Aged Quality of life Fractures Bone Fracture Fixation Bone Plates Fracture Fixation Internal Randomized Controlled Trials as Topic Multicenter Studies as Topic
In vivo visualization of age-related differences in the locus coeruleus.
Kathy Y Liu, Julio Acosta-Cabronero, Arturo Cardenas-Blanco, Clare Loane, Alex J Berry, Matthew J Betts, Rogier A Kievit, Richard N Henson, Emrah Düzel, Cam-CAN, Robert Howard, Dorothea Hämmerer
Dec 18, 2018
The locus coeruleus (LC), the major origin of noradrenergic modulation of the central nervous system, may play an important role in neuropsychiatric disorders including Parkinson's disease and Alzheimer's disease. The pattern of...
Noradrenergic-dependent functions are associated with age-related locus coeruleus signal intensity differences
Kathy Y. Liu, Rogier A. Kievit, Kamen A. Tsvetanov, Matthew J. Betts, Emrah Düzel, James B. Rowe, Robert Howard, Dorothea Hämmerer, Lorraine K. Tyler, Carol Brayne, Edward T. Bullmore, Andrew C. Calder, Rhodri Cusack, Tim Dalgleish, John Duncan, Richard N. Henson, Fiona E. Matthews, William D. Marslen-Wilson, Meredith A. Shafto, Karen Campbell, Teresa Cheung, Simon Davis, Linda Geerligs, Rogier Kievit, Anna McCarrey, Abdur Mustafa, Darren Price, David Samu, Jason R. Taylor, Matthias Treder, Janna van Belle, Nitin Williams, Lauren Bates, Tina Emery, Sharon Erzinçlioglu, Andrew Gadie, Sofia Gerbase, Stanimira Georgieva, Claire Hanley, Beth Parkin, David Troy, Tibor Auer, Marta Correia, Lu Gao, Emma Green, Rafael Henriques, Jodie Allen, Gillian Amery, Liana Amunts, Anne Barcroft, Amanda Castle, Cheryl Dias, Jonathan Dowrick, Melissa Fair, Hayley Fisher, Anna Goulding, Adarsh Grewal, Geoff Hale, Andrew Hilton, Frances Johnson, Patricia Johnston, Thea Kavanagh-Williamson, Magdalena Kwasniewska, Alison McMinn, Kim Norman, Jessica Penrose, Fiona Roby, Diane Rowland, John Sargeant, Maggie Squire, Beth Stevens, Aldabra Stoddart, Cheryl Stone, Tracy Thompson, Ozlem Yazlik, Dan Barnes, Marie Dixon, Jaya Hillman, Joanne Mitchell, Laura Villis
Apr 06, 2021
Abstract: The locus coeruleus (LC), the origin of noradrenergic modulation of cognitive and behavioral function, may play an important role healthy ageing and in neurodegenerative conditions. We investigated the functional...
Lateral compression type 1 fracture fixation in the elderly (L1FE)
Elizabeth Cook, Joanne Laycock, Mehool Acharya, Michael Ross Backhouse, Belen Corbacho, Laura Doherty, Daren Forward, Catherine Hewitt, Catherine Hilton, Peter Hull, Jamila Kassam, Camila Maturana, Catriona Mcdaid, Jenny Roche, Dhanupriya Sivapathasuntharam, David Torgerson, Peter Bates
Feb 02, 2023
BACKGROUND: Lateral compression type1 (LC-1) fragility fractures are a common, painful injury in older adults resulting in reduced mobility. The incidence of these fractures is increasing with the growing older adult population....
Clinical Utility of microRNAs in Exhaled Breath Condensate as Biomarkers for Lung Cancer
Carlos Pérez-Sánchez, Nuria Barbarroja, Lucas C. Pantaleão, Laura M. López-Sánchez, Susan E. Ozanne, Bernabé Jurado-Gámez, Enrique Aranda, Chary Lopez-Pedrera, Antonio Rodríguez-Ariza
Feb 10, 2021
This study represents a novel proof of concept of the clinical utility of miRNAs from exhaled breath condensate (EBC) as biomarkers of lung cancer (LC). Genome-wide miRNA profiling and machine learning analysis were performed on...
Some more on the history of present-tense -s, do and zero
LC Wright
Apr 10, 2017
This paper analyses the present indicative third-person singular markers in a diary kept by a servant from West Oxfordshire in 1837. There are three present-tense markers present in the diary: periphrastic auxiliary verb do, -s...
Multilevel quasi-Monte Carlo methods for lognormal diffusion problems
In this paper we present a rigorous cost and error analysis of amultilevel estimator based on randomly shifted Quasi-Monte Carlo (QMC)lattice rules for lognormal diffusion problems. These problems are motivatedby uncertainty...
|
|< |
< |
1 |