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Results: 19631
On the origin of the human germline.
In mice, primordial germ cells (PGCs), the precursors of eggs and sperm, originate from pregastrulation postimplantation embryos. By contrast, the origin of human PGCs (hPGCs) has been less clear and has been difficult to study...
Amnion Epiblast Epigenetic resetting Gastrulation human development primordial germ cells Signalling Transcription Factors Animals Cell Differentiation Embryo Mammalian Embryonic Development Embryonic Stem Cells Epigenesis Genetic Gene Regulatory Networks Germ Cells Germ Layers Human Embryonic Stem Cells Humans Mice Pluripotent Stem Cells
The unfolding body plan of primate embryos in culture.
Improved culture of non-human primate embryos reveals the establishment of the crucial framework for subsequent development of bodily tissues and the germline in fetuses, bringing us closer to comprehending the elusive...
Germ cell specification and pluripotency in mammals
Germ cells are unique cell types that generate a totipotent zygote upon fertilization, giving rise to the next generation in mammals and many other multicellular organisms. How germ cells acquire this ability has been of...
In retrospect
M Azim Surani
Nov 27, 2019
Pluripotent cells have the potential to differentiate into any cell type in the body. Their isolation and propagation from mouse embryos was pivotal for advances in understanding human development and disease.
Conserved features of non-primate bilaminar disc embryos and the germline.
Post-implantation embryo development commences with a bilaminar disc in most mammals, including humans. Whereas access to early human embryos is limited and subject to greater ethical scrutiny, studies on non-primate embryos...
Developmental Competence for Primordial Germ Cell Fate.
During mammalian embryonic development, the trophectoderm and primitive endoderm give rise to extraembryonic tissues, while the epiblast differentiates into all somatic lineages and the germline. Remarkably, only a few classes...
Specification and epigenetic programming of the human germ line.
Primordial germ cells (PGCs), the precursors of sperm and eggs, are established in perigastrulation-stage embryos in mammals. Signals from extra-embryonic tissues induce a unique gene regulatory network in germline-competent...
Testing the role of SOX15 in human primordial germ cell fate.
Background: Potentially novel regulators of early human germline development have been identified recently, including SOX15 and SOX17, both of which show specific expression in human primordial germ cells. SOX17 is now known to...
Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development.
Jan J Zylicz, Sabine Dietmann, Ufuk Günesdogan, Jamie A Hackett, Delphine Cougot, Caroline Lee, M Azim Surani
Nov 24, 2015
Chromosomes developmental biology Epiblast epigenetics Genes histone MOUSE post-implantation primed pluripotency Stem Cells Animals Chromatin Embryonic Development Enhancer of Zeste Homolog 2 Protein Gene Expression Regulation Developmental Histone-Lysine N-Methyltransferase Histones Methylation Mice Polycomb Repressive Complex 2 Protein Processing Post-Translational
A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons
Anastasiya Sybirna, Walfred W. C. Tang, Merrick Pierson Smela, Sabine Dietmann, Wolfram H. Gruhn, Ran Brosh, M. Azim Surani
Mar 09, 2021
A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons.
Anastasiya Sybirna, Walfred WC Tang, Merrick Pierson Smela, Sabine Dietmann, Wolfram H Gruhn, Ran Brosh, M Azim Surani
Jan 15, 2020
A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons.
Anastasiya Sybirna, Walfred W C Tang, Merrick Pierson Smela, Sabine Dietmann, Wolfram H Gruhn, Ran Brosh, M Azim Surani
Apr 11, 2020
PRDM14 is a crucial regulator of mouse primordial germ cells (mPGCs), epigenetic reprogramming and pluripotency, but its role in the evolutionarily divergent regulatory network of human PGCs (hPGCs) remains unclear. Besides, a...
Published by: Nature Communications
SOX17 is a critical specifier of human primordial germ cell fate.
Naoko Irie, Leehee Weinberger, Walfred WC Tang, Toshihiro Kobayashi, Sergey Viukov, Yair S Manor, Sabine Dietmann, Jacob H Hanna, M Azim Surani
Mar 20, 2015
NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers.
Kazuhiro Murakami, Ufuk Günesdogan, Jan J Zylicz, Walfred WC Tang, Roopsha Sengupta, Toshihiro Kobayashi, Shinseog Kim, Richard Butler, Sabine Dietmann, M Azim Surani
Dec 09, 2015
Activins Animals Bone Morphogenetic Protein 4 Cell Differentiation Chromatin DNA-Binding Proteins Enhancer Elements Genetic Epigenesis Genetic Female Fibroblast Growth Factor 2 Gene Expression Regulation Developmental Genome Germ Cells Germ Layers Homeodomain Proteins Male Mice Mouse embryonic stem cells Nanog Homeobox Protein Pluripotent Stem Cells Positive Regulatory Domain I-Binding Factor 1 Protein Binding RNA-Binding Proteins SOXB1 Transcription Factors Transcription Factor AP-2 Transcription Factors
Esrrb Complementation Rescues Development of Nanog-Null Germ Cells.
Man Zhang, Harry G Leitch, Walfred WC Tang, Nicola Festuccia, Elisa Hall-Ponsele, Jennifer Nichols, M Azim Surani, Austin Smith, Ian Chambers
Mar 19, 2018
The transcription factors (TFs) Nanog and Esrrb play important roles in embryonic stem cells (ESCs) and during primordial germ-cell (PGC) development. Esrrb is a positively regulated direct target of NANOG in ESCs that can...
PRMT5 protects genomic integrity during global DNA demethylation in primordial germ cells and preimplantation embryos.
Shinseog Kim, Ufuk Günesdogan, Jan J Zylicz, Jamie A Hackett, Delphine Cougot, Siqin Bao, Caroline Lee, Sabine Dietmann, George E Allen, Roopsha Sengupta, M Azim Surani
Apr 14, 2015
Animals Apoptosis Blastocyst Cells Cultured DNA Damage DNA Methylation DNA Transposable Elements Embryonic Development Embryonic Stem Cells Female Genomic Instability Histones Male Mice Transgenic Ovum Protein Methyltransferases Protein Processing Post-Translational Protein-Arginine N-Methyltransferases Spermatozoa
Blastocyst complementation using Prdm14 -deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats
Toshihiro Kobayashi, Teppei Goto, Mami Oikawa, Makoto Sanbo, Fumika Yoshida, Reiko Terada, Naoko Niizeki, Naoyo Kajitani, Kanako Kazuki, Yasuhiro Kazuki, Shinichi Hochi, Hiromitsu Nakauchi, M. Azim Surani, Masumi Hirabayashi
Feb 26, 2021
Abstract: Murine animal models from genetically modified pluripotent stem cells (PSCs) are essential for functional genomics and biomedical research, which require germline transmission for the establishment of colonies....
G9a regulates temporal preimplantation developmental program and lineage segregation in blastocyst
Jan J Zylicz, Maud Borensztein, Frederick CK Wong, Yun Huang, Caroline Lee, Sabine Dietmann, Azim Surani
Sep 28, 2018
DNMTs Play an Important Role in Maintaining the Pluripotency of Leukemia Inhibitory Factor-Dependent Embryonic Stem Cells.
Baojiang Wu, Yunxia Li, Bojiang Li, Baojing Zhang, Yanqiu Wang, Lin Li, Junpeng Gao, Yuting Fu, Shudong Li, Chen Chen, M Azim Surani, Fuchou Tang, Xihe Li, Siqin Bao
Apr 16, 2021
Naive pluripotency can be maintained in medium with two inhibitors plus leukemia inhibitory factor (2i/LIF) supplementation, which primarily affects canonical WNT, FGF/ERK, and JAK/STAT3 signaling. However, whether one of these...
DNA Methylation DNMTs DIFFERENTIATION Embryonic Stem Cells epigenetic Genomic Imprinting Leukemia Inhibitory Factor MOUSE Pluripotency self-renew Animals Cell Culture Techniques Cell Differentiation Cell Self Renewal Cells Cultured Culture Media DNA (Cytosine-5-)-Methyltransferases DNA Methylation DNA Methyltransferase 3A Gene Expression Regulation Developmental Gene Knockout Techniques Genomic Imprinting Germ Layers Janus Kinases Leukemia Inhibitory Factor Mice Mice Inbred C57BL Mice Transgenic Mouse embryonic stem cells STAT3 Transcription Factor Signal Transduction Transcriptome
Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis.
Priscila Ramos-Ibeas, Fei Sang, Qifan Zhu, Walfred WC Tang, Sarah Withey, Doris Klisch, Liam Wood, Matt Loose, M Azim Surani, Ramiro Alberio
Apr 09, 2019
High-resolution molecular programmes delineating the cellular foundations of mammalian embryogenesis have emerged recently. Similar analysis of human embryos is limited to pre-implantation stages, since early post-implantation...
Targeted DamID reveals differential binding of mammalian pluripotency factors.
Seth W Cheetham, Wolfram H Gruhn, Jelle van den Ameele, Robert Krautz, Tony D Southall, Toshihiro Kobayashi, M Azim Surani, Andrea H Brand
Oct 03, 2018
The precise control of gene expression by transcription factor networks is crucial to organismal development. The predominant approach for mapping transcription factor-chromatin interactions has been chromatin...
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