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High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology.
Laetitia Chauve, Jérémie Le Pen, Francesca Hodge, Pia Todtenhaupt, Laura Biggins, Eric A Miska, Simon Andrews, Olivia Casanueva
Jun 22, 2020
This paper presents a high-throughput reverse transcription quantitative PCR (RT-qPCR) assay for Caenorhabditis elegans that is fast, robust, and highly sensitive. This protocol obtains precise measurements of gene expression...
Measurement delay associated with the Guardian RT continuous glucose monitoring system.
AIMS: Using compartment modelling, we assessed the time delay between blood glucose and sensor glucose measured by the Guardian RT continuous glucose monitoring system in young subjects with Type 1 diabetes (T1D). METHODS...
Prevalence of RT-qPCR-detected SARS-CoV-2 infection at schools
Peter Willeit, Robert Krause, Bernd Lamprecht, Andrea Berghold, Buck Hanson, Evelyn Stelzl, Heribert Stoiber, Johannes Zuber, Robert Heinen, Alwin Köhler, David Bernhard, Wegene Borena, Christian Doppler, Dorothee von Laer, Hannes Schmidt, Johannes Pröll, Ivo Steinmetz, Michael Wagner
Sep 16, 2021
BACKGROUND: The role of schools in the SARS-CoV-2 pandemic is much debated. We aimed to quantify reliably the prevalence of SARS-CoV-2 infections at schools detected with reverse-transcription quantitative...
Design, development, and validation of a strand-specific RT-qPCR assay for GI and GII human Noroviruses.
Katja Marie Kjara König, Aminu S Jahun, Komal Nayak, Lydia N Drumright, Matthias Zibauer, Ian Goodfellow, Myra Hosmillo
Jan 06, 2022
Human noroviruses (HuNoV) are the major cause of viral gastroenteritis worldwide. Similar to other positive-sense single-stranded RNA viruses, norovirus RNA replication requires the formation of a negative strand RNA...
Design, development, and validation of a strand-specific RT-qPCR assay for GI and GII human Noroviruses.
Katja Marie Kjara König, Aminu Jahun, Komal Nayak, Lydia N Drumright, Matthias Zibauer, Ian Goodfellow, Myra Hosmillo
Nov 03, 2021
Human noroviruses (HuNoV) are the major cause of viral gastroenteritis worldwide. Similar to other positive-sense single-stranded RNA viruses, norovirus RNA replication requires the formation of a negative strand RNA...
Patterns of the use of advanced radiation therapy techniques for the management of bone metastases and the associated factors in Victoria.
Tamara Fogarty, Mark Tacey, Giulia McCorkell, David Kok, Colin Hornby, Roger L Milne, Jeremy Millar, Farshad Foroudi, Wee Loon Ong
Feb 02, 2022
INTRODUCTION: To describe the pattern of the use of advanced radiation therapy (RT) techniques, including intensity-modulated RT (IMRT), volumetric modulated arc therapy (VMAT), and stereotactic body RT (SBRT) for the management...
Impact of Irradiation on the Pharmacokinetics and Biotransformation of Tamoxifen.
Yung-Yi Cheng, Teresa Zheng, Michael W Chang, Jeffrey W Dalley, Yu-Jen Chen, Tung-Hu Tsai, Chen-Hsi Hsieh
Mar 03, 2022
BACKGROUND: The optimal procedure for combining radiotherapy (RT) with tamoxifen treatment is controversial as RT may alter the pharmacokinetics and biotransformation of tamoxifen. The present study investigated this potential...
Contribution of retrotransposition to developmental disorders.
Eugene J Gardner, Elena Prigmore, Giuseppe Gallone, Petr Danecek, Kaitlin E Samocha, Juliet Handsaker, Sebastian S Gerety, Holly Ironfield, Patrick J Short, Alejandro Sifrim, Tarjinder Singh, Kate E Chandler, Emma Clement, Katherine L Lachlan, Katrina Prescott, Elisabeth Rosser, David R FitzPatrick, Helen V Firth, Matthew E Hurles
Oct 10, 2020
Mobile genetic Elements (MEs) are segments of DNA which can copy themselves and other transcribed sequences through the process of retrotransposition (RT). In humans several disorders have been attributed to RT, but the role of...
Large variation in radiation therapy fractionation for multiple myeloma in Australia.
AIM: To evaluate the patterns of use of different radiation therapy (RT) fractionation for multiple myeloma (MM) bone disease. METHODS: This is a population-based cohort of patients with MM who had RT between 2012 and 2017 as...
Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia.
Ursula Panzner, Ondari Daniel Mogeni, Yaw Adu-Sarkodie, Trevor Toy, Hyon Jin Jeon, Gi Deok Pak, Se Eun Park, Yeetey Enuameh, Ellis Owusu-Dabo, Trinh Van Tan, Abraham Aseffa, Mekonnen Teferi, Biruk Yeshitela, Stephen Baker, Raphael Rakotozandrindrainy, Florian Marks
Oct 02, 2022
BACKGROUND: We aimed to assess the prevalence of Salmonella Typhi through DNA and IgM-antibody detection methods as a prelude to extended surveillance activities at sites in Ghana, Madagascar, and Ethiopia. METHODS: We performed...
ELISA febrile illness HlyE-/CdtB-/PilL-/Vi-antigen salmonella SURVEILLANCE Adolescent Corneal Dystrophies Hereditary Enzyme-Linked Immunosorbent Assay Ethiopia Female Fever Ghana Humans Immunoglobulin M Madagascar Male Nucleic Acids Reverse Transcriptase Polymerase Chain Reaction salmonella Salmonella typhi Typhoid Fever
Wave runup video detection using the Radon Transform
Rafael Almar, Christopher Blenkinsopp, Luis Pedro Almeida, Rodrigo Cienfuegos, P Catalan
Dec 01, 2017
A new method of runup detection from video imagery is introduced and validated at an energetic dissipative beach. The instantaneous waterline is detected from uprush and backwash by using the Radon Transform (RT). The method is...
Automated customized retrieval of radiotherapy data for clinical trials, audit and research.
Marina Romanchikova, Karl Harrison, Neil G Burnet, Andrew CF Hoole, Michael PF Sutcliffe, Michael Andrew Parker, Rajesh Jena, Simon James Thomas
Feb 07, 2018
OBJECTIVE: To enable fast and customizable automated collection of radiotherapy (RT) data from tomotherapy storage. METHODS: Human-readable data maps (TagMaps) were created to generate DICOM-RT (Digital Imaging and...
Wave runup video detection using the Radon Transform
Rafael Almar, Christopher Blenkinsopp, Luis Pedro Almeida, Rodrigo Cienfuegos, P Catalan
Dec 01, 2017
A new method of runup detection from video imagery is introduced and validated at an energetic dissipative beach. The instantaneous waterline is detected from uprush and backwash by using the Radon Transform (RT). The method is...
A Distributed Spatial Index With High Update Efficiency for Location-Based Real-Time Services
LBS-RT (location-based service in a real-time manner) has become popular because it can provide quick and timely services. Range query is often used in LBS-RT, which finds objects in a specified area, and spatial indices are...
Published by: IGI Global Scientific Publishing
A case-control study of the joint effect of reproductive factors and radiation treatment for first breast cancer and risk of contralateral breast cancer in the WECARE study.
Jennifer D Brooks, John D Boice, Roy E Shore, Anne S Reiner, Susan A Smith, Leslie Bernstein, Julia A Knight, Charles F Lynch, Esther M John, Kathleen E Malone, Lene Mellemkjær, Rikke Langballe, Xiaolin Liang, Meghan Woods, Marc Tischkowitz, Patrick Concannon, Daniel O Stram, WECARE Study Collaborative Group
Oct 23, 2020
OBJECTIVE: To examined the impact of reproductive factors on the relationship between radiation treatment (RT) for a first breast cancer and risk of contralateral breast cancer (CBC). METHODS: The Women's Environmental Cancer...
AI-Assisted CT as a Clinical and Research Tool for COVID-19.
Zion Tsz Ho Tse, Sierra Hovet, Hongliang Ren, Tristan Barrett, Sheng Xu, Baris Turkbey, Bradford J Wood
Sep 08, 2021
There is compelling support for widening the role of computed tomography (CT) for COVID-19 in clinical and research scenarios. Reverse transcription polymerase chain reaction (RT-PCR) testing, the gold standard for COVID-19...
Published by: Frontiers in artificial intelligence
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