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Cover Image for High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology. View Article
High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology.
Laetitia Chauve, Jérémie Le Pen, Francesca Hodge, Pia Todtenhaupt, Laura Biggins, Eric A Miska, Simon Andrews, Olivia Casanueva
Jun 22, 2020
This paper presents a high-throughput reverse transcription quantitative PCR (RT-qPCR) assay for Caenorhabditis elegans that is fast, robust, and highly sensitive. This protocol obtains precise measurements of gene expression...
Animals Caenorhabditis elegans Caenorhabditis elegans Proteins DNA Primers DNA Complementary DNA Helminth HEAT-SHOCK PROTEINS Oligonucleotide Array Sequence Analysis RNA Messenger Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction
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Cover Image for Measurement delay associated with the Guardian RT continuous glucose monitoring system. View Article
Measurement delay associated with the Guardian RT continuous glucose monitoring system.
C Wei, DJ Lunn, CL Acerini, JM Allen, AM Larsen, ME Wilinska, DB Dunger, R Hovorka
Feb 21, 2020
AIMS: Using compartment modelling, we assessed the time delay between blood glucose and sensor glucose measured by the Guardian RT continuous glucose monitoring system in young subjects with Type 1 diabetes (T1D). METHODS...
Adolescent Blood Glucose Blood Glucose Self-Monitoring Body Mass Index Child Diabetes mellitus Type 1 Female Humans Male Reproducibility of Results Time Factors
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Cover Image for Multi-dimensional super-resolution imaging enables surface hydrophobicity mapping View Article
Multi-dimensional super-resolution imaging enables surface hydrophobicity mapping
MN Bongiovanni, J Godet, MH Horrocks, L Tosatto, AR Carr, DC Wirthensohn, RT Ranasinghe, JV Fritz, CM Dobson, D Klenerman, SF Lee
Dec 06, 2016
0299 Other Physical Sciences 0601 Biochemistry and Cell Biology Generic Health Relevance
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Cover Image for Prevalence of RT-qPCR-detected SARS-CoV-2 infection at schools View Article
Prevalence of RT-qPCR-detected SARS-CoV-2 infection at schools
Peter Willeit, Robert Krause, Bernd Lamprecht, Andrea Berghold, Buck Hanson, Evelyn Stelzl, Heribert Stoiber, Johannes Zuber, Robert Heinen, Alwin Köhler, David Bernhard, Wegene Borena, Christian Doppler, Dorothee von Laer, Hannes Schmidt, Johannes Pröll, Ivo Steinmetz, Michael Wagner
Sep 16, 2021
BACKGROUND: The role of schools in the SARS-CoV-2 pandemic is much debated. We aimed to quantify reliably the prevalence of SARS-CoV-2 infections at schools detected with reverse-transcription quantitative...
Emerging Infectious Diseases Infectious Diseases Prevention 3 Good Health and Well Being
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Cover Image for Design, development, and validation of a strand-specific RT-qPCR assay for GI and GII human Noroviruses. View Article
Design, development, and validation of a strand-specific RT-qPCR assay for GI and GII human Noroviruses.
Katja Marie Kjara König, Aminu S Jahun, Komal Nayak, Lydia N Drumright, Matthias Zibauer, Ian Goodfellow, Myra Hosmillo
Jan 06, 2022
Human noroviruses (HuNoV) are the major cause of viral gastroenteritis worldwide. Similar to other positive-sense single-stranded RNA viruses, norovirus RNA replication requires the formation of a negative strand RNA...
HuNoV replicon RT-qPCR calicivirus Human Intestinal Organoids human norovirus intestinal epithelial cells strand-specific RT-qPCR viral gastroenteritis
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Cover Image for Design, development, and validation of a strand-specific RT-qPCR assay for GI and GII human Noroviruses. View Article
Design, development, and validation of a strand-specific RT-qPCR assay for GI and GII human Noroviruses.
Katja Marie Kjara König, Aminu Jahun, Komal Nayak, Lydia N Drumright, Matthias Zibauer, Ian Goodfellow, Myra Hosmillo
Nov 03, 2021
Human noroviruses (HuNoV) are the major cause of viral gastroenteritis worldwide. Similar to other positive-sense single-stranded RNA viruses, norovirus RNA replication requires the formation of a negative strand RNA...
HuNoV replicon RT-qPCR calicivirus Human Intestinal Organoids human norovirus intestinal epithelial cells strand-specific RT-qPCR viral gastroenteritis
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Cover Image for Evaluating the effects of resources and solvability on burglary detection View Article
Evaluating the effects of resources and solvability on burglary detection
RT Coupe
Jan 26, 2015
resources solvability detection clearance
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Cover Image for Patterns of the use of advanced radiation therapy techniques for the management of bone metastases and the associated factors in Victoria. View Article
Patterns of the use of advanced radiation therapy techniques for the management of bone metastases and the associated factors in Victoria.
Tamara Fogarty, Mark Tacey, Giulia McCorkell, David Kok, Colin Hornby, Roger L Milne, Jeremy Millar, Farshad Foroudi, Wee Loon Ong
Feb 02, 2022
INTRODUCTION: To describe the pattern of the use of advanced radiation therapy (RT) techniques, including intensity-modulated RT (IMRT), volumetric modulated arc therapy (VMAT), and stereotactic body RT (SBRT) for the management...
IMRT VMAT bone metastases Radiation therapy stereotactic Aged Humans Male Medicare Radiosurgery Radiotherapy Dosage Radiotherapy Planning Computer-Assisted Radiotherapy Intensity-Modulated Spinal Neoplasms United States
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Cover Image for Individual Differences in Theory of Mind From Preschool to Adolescence View Article
Individual Differences in Theory of Mind From Preschool to Adolescence
C Hughes, RT Devine
Jul 30, 2015
Theory of mind individual differences longitudinal social development
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Cover Image for Does Parental Mind-Mindedness Account for Cross-Cultural Differences in Preschoolers' Theory of Mind? View Article
Does Parental Mind-Mindedness Account for Cross-Cultural Differences in Preschoolers' Theory of Mind?
C Hughes, RT Devine, Z Wang
Mar 08, 2017
Child Preschool Cross-Cultural Comparison Female Hong Kong Humans Male Parent-Child Relations Parents Theory of mind United Kingdom
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Cover Image for Impact of Irradiation on the Pharmacokinetics and Biotransformation of Tamoxifen. View Article
Impact of Irradiation on the Pharmacokinetics and Biotransformation of Tamoxifen.
Yung-Yi Cheng, Teresa Zheng, Michael W Chang, Jeffrey W Dalley, Yu-Jen Chen, Tung-Hu Tsai, Chen-Hsi Hsieh
Mar 03, 2022
BACKGROUND: The optimal procedure for combining radiotherapy (RT) with tamoxifen treatment is controversial as RT may alter the pharmacokinetics and biotransformation of tamoxifen. The present study investigated this potential...
Breast Cancer Pharmacokinetics Radiotherapy Tamoxifen Tandem Mass Spectrometry
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Cover Image for Super-resolution imaging reveals α-synuclein seeded aggregation in SH-SY5Y cells. View Article
Super-resolution imaging reveals α-synuclein seeded aggregation in SH-SY5Y cells.
Jason C Sang, Eric Hidari, Georg Meisl, Rohan T Ranasinghe, Maria Grazia Spillantini, David Klenerman
Aug 22, 2022
Amyloid Humans Molecular Imaging Neuroblastoma Protein Aggregates Tumor Cells Cultured ALPHA-SYNUCLEIN
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Cover Image for Contribution of retrotransposition to developmental disorders. View Article
Contribution of retrotransposition to developmental disorders.
Eugene J Gardner, Elena Prigmore, Giuseppe Gallone, Petr Danecek, Kaitlin E Samocha, Juliet Handsaker, Sebastian S Gerety, Holly Ironfield, Patrick J Short, Alejandro Sifrim, Tarjinder Singh, Kate E Chandler, Emma Clement, Katherine L Lachlan, Katrina Prescott, Elisabeth Rosser, David R FitzPatrick, Helen V Firth, Matthew E Hurles
Oct 10, 2020
Mobile genetic Elements (MEs) are segments of DNA which can copy themselves and other transcribed sequences through the process of retrotransposition (RT). In humans several disorders have been attributed to RT, but the role of...
Developmental Disabilities genetic variation Humans Mutation Rate Retroelements
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Cover Image for Large variation in radiation therapy fractionation for multiple myeloma in Australia. View Article
Large variation in radiation therapy fractionation for multiple myeloma in Australia.
Wee Loon Ong, Michael MacManus, Roger L Milne, Farshad Foroudi, Jeremy L Millar
May 23, 2022
AIM: To evaluate the patterns of use of different radiation therapy (RT) fractionation for multiple myeloma (MM) bone disease. METHODS: This is a population-based cohort of patients with MM who had RT between 2012 and 2017 as...
Humans Dose Fractionation Radiation multiple myeloma Bone Neoplasms Radiation Oncology Palliative Care Australia
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Cover Image for Tourism-Driven Mobilities View Article
Tourism-Driven Mobilities
Ruwan Ranasinghe, Li Cheng
Jul 01, 2020
Mobilities discourse, regardless of its vital significance in day-to-day life, is under examined. It draws researchers' attention at theoretical level showing significant absence of empirical studies. The absence of a scale to...
Global Business Business and Management Global Business
Published by: IGI Global Scientific Publishing
Cover Image for For Better or for Worse? Positive and Negative Parental Influences on Young Children's Executive Function View Article
For Better or for Worse? Positive and Negative Parental Influences on Young Children's Executive Function
C Hughes, RT Devine
Jul 17, 2017
Adult Child Child Development Child Preschool Executive Function Female Humans language learning Longitudinal Studies Male Parent-Child Relations Parenting Parents
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Cover Image for Improving the lateral load resistance of vernacular masonry walls subject to flooding View Article
Improving the lateral load resistance of vernacular masonry walls subject to flooding
Shawn Platt, Indunil Erandi, Chintha Jayasinghe, Thishan Jayasinghe, Dan Maskell, Gihan Ranasinghe, Pete Walker
May 31, 2023
Low rise vernacular masonry buildings are vulnerable to damage from extreme flooding. This paper presents findings from a study based in Sri Lanka assessing the structural resilience of non-engineered single storey houses...
buildings structures & design Developing Countries floods & floodworks /dk/atira/pure/subjectarea/asjc/2200/2205 name=Civil and Structural Engineering /dk/atira/pure/subjectarea/asjc/2500/2500 name=General Materials Science /dk/atira/pure/subjectarea/asjc/2200/2211 name=Mechanics of Materials
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Cover Image for Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia. View Article
Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia.
Ursula Panzner, Ondari Daniel Mogeni, Yaw Adu-Sarkodie, Trevor Toy, Hyon Jin Jeon, Gi Deok Pak, Se Eun Park, Yeetey Enuameh, Ellis Owusu-Dabo, Trinh Van Tan, Abraham Aseffa, Mekonnen Teferi, Biruk Yeshitela, Stephen Baker, Raphael Rakotozandrindrainy, Florian Marks
Oct 02, 2022
BACKGROUND: We aimed to assess the prevalence of Salmonella Typhi through DNA and IgM-antibody detection methods as a prelude to extended surveillance activities at sites in Ghana, Madagascar, and Ethiopia. METHODS: We performed...
ELISA febrile illness HlyE-/CdtB-/PilL-/Vi-antigen salmonella SURVEILLANCE Adolescent Corneal Dystrophies Hereditary Enzyme-Linked Immunosorbent Assay Ethiopia Female Fever Ghana Humans Immunoglobulin M Madagascar Male Nucleic Acids Reverse Transcriptase Polymerase Chain Reaction salmonella Salmonella typhi Typhoid Fever
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Cover Image for The role of vegetation cover and slope angle in tephra layer preservation and implications for Quaternary tephrostratigraphy View Article
The role of vegetation cover and slope angle in tephra layer preservation and implications for Quaternary tephrostratigraphy
AJ Dugmore, RT Streeter, NA Cutler
Feb 05, 2018
Grímsvötn Eyjafjallajökull Mount St Helens biocrust isopach maps fallout volumes
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Cover Image for Is there an optimal preoperative management strategy for phaeochromocytoma/paraganglioma? View Article
Is there an optimal preoperative management strategy for phaeochromocytoma/paraganglioma?
BG Challis, RT Casey, HL Simpson, M Gurnell
Dec 08, 2016
Adrenergic alpha-Antagonists Humans Paraganglioma Pheochromocytoma Postoperative Complications Preoperative Care
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Cover Image for Wave runup video detection using the Radon Transform View Article
Wave runup video detection using the Radon Transform
Rafael Almar, Christopher Blenkinsopp, Luis Pedro Almeida, Rodrigo Cienfuegos, P Catalan
Dec 01, 2017
A new method of runup detection from video imagery is introduced and validated at an energetic dissipative beach. The instantaneous waterline is detected from uprush and backwash by using the Radon Transform (RT). The method is...
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Cover Image for Automated customized retrieval of radiotherapy data for clinical trials, audit and research. View Article
Automated customized retrieval of radiotherapy data for clinical trials, audit and research.
Marina Romanchikova, Karl Harrison, Neil G Burnet, Andrew CF Hoole, Michael PF Sutcliffe, Michael Andrew Parker, Rajesh Jena, Simon James Thomas
Feb 07, 2018
OBJECTIVE: To enable fast and customizable automated collection of radiotherapy (RT) data from tomotherapy storage. METHODS: Human-readable data maps (TagMaps) were created to generate DICOM-RT (Digital Imaging and...
Automation Clinical Trials as Topic Humans Information Storage and Retrieval Management Audit Radiology Information Systems Radiotherapy Software
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Cover Image for A Distributed Spatial Index With High Update Efficiency for Location-Based Real-Time Services View Article
A Distributed Spatial Index With High Update Efficiency for Location-Based Real-Time Services
Junhua Fang, Zonglei Zhang
Jan 01, 2023
LBS-RT (location-based service in a real-time manner) has become popular because it can provide quick and timely services. Range query is often used in LBS-RT, which finds objects in a specified area, and spatial indices are...
Databases Computer Science and Information Technology Data Mining and Databases
Published by: IGI Global Scientific Publishing
Cover Image for Parochial cooperation in wild chimpanzees View Article
Parochial cooperation in wild chimpanzees
Sylvain RT Lemoine, Liran Samuni, Catherine Crockford, Roman M Wittig
Apr 06, 2022
ARTICLES Review articles Human evolution Primates Social ties Oxytocin in-group out-group parochial cooperation model
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Cover Image for Wave runup video detection using the Radon Transform View Article
Wave runup video detection using the Radon Transform
Rafael Almar, Christopher Blenkinsopp, Luis Pedro Almeida, Rodrigo Cienfuegos, P Catalan
Dec 01, 2017
A new method of runup detection from video imagery is introduced and validated at an energetic dissipative beach. The instantaneous waterline is detected from uprush and backwash by using the Radon Transform (RT). The method is...
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