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Results: 30
Tia1 dependent regulation of mRNA subcellular location and translation controls p53 expression in B cells.
Manuel D Díaz-Muñoz, Vladimir Yu Kiselev, Nicolas Le Novère, Tomaz Curk, Jernej Ule, Martin Turner
Jun 24, 2019
Post-transcriptional regulation of cellular mRNA is essential for protein synthesis. Here we describe the importance of mRNA translational repression and mRNA subcellular location for protein expression during B lymphocyte...
Modeling Disease with Human Inducible Pluripotent Stem Cells.
Understanding the physiopathology of disease remains an essential step in developing novel therapeutics. Although animal models have certainly contributed to advancing this enterprise, their limitation in modeling all the...
Optimal multivalent targeting of membranes with many distinct receptors
Cells can often be recognized by the concentrations of receptors expressed on their surface. For better (targeted drug treatment) or worse (targeted infection by pathogens), it is clearly important to be able to target cells...
Nanoparticle organization in sandwiched polymer brushes.
The organization of nanoparticles inside grafted polymer layers is governed by the interplay of polymer-induced entropic interactions and the action of externally applied fields. Earlier work had shown that strong external...
Crystallinity of Double-Stranded RNA-Antimicrobial Peptide Complexes Modulates Toll-Like Receptor 3-Mediated Inflammation.
Ernest Y Lee, Toshiya Takahashi, Tine Curk, Jure Dobnikar, Richard L Gallo, Gerard CL Wong
Jan 22, 2018
Double-stranded RNA (dsRNA) induces production of pro-inflammatory cytokines in normal human epidermal keratinocytes (NHEK) by specific binding to endosomal Toll-like receptor-3 (TLR3). Recently, it has been shown that...
The Effect of Attractive Interactions and Macromolecular Crowding on Crystallins Association.
In living systems proteins are typically found in crowded environments where their effective interactions strongly depend on the surrounding medium. Yet, their association and dissociation needs to be robustly controlled in...
Identifying genetic variants that affect viability in large cohorts
Hakhamanesh Mostafavi, Tomaz Berisa, Felix R Day, John RB Perry, Molly Przeworski, Joseph K Pickrell
Jan 15, 2018
Analysis of CLIP and iCLIP methods for nucleotide-resolution studies of protein-RNA interactions.
Yoichiro Sugimoto, Julian König, Shobbir Hussain, Blaž Zupan, Tomaž Curk, Michaela Frye, Jernej Ule
Jan 02, 2013
Animals Antigens Neoplasm COS Cells Chlorocebus aethiops Gene Library HeLa Cells High-Throughput Nucleotide Sequencing Humans Immunoprecipitation Methyltransferases Mice Molecular Sequence Annotation Nerve Tissue Proteins Neuro-Oncological Ventral Antigen RNA RNA-Binding Proteins Sequence Analysis DNA Sequence Deletion
Analysis of CLIP and iCLIP methods for nucleotide-resolution studies of protein-RNA interactions
Yoichiro Sugimoto, Julian König, Shobbir Hussain, Blaž Zupan, Tomaž Curk, Michaela Frye, Jernej Ule
Jan 01, 0001
UV cross-linking and immunoprecipitation (CLIP) and individual-nucleotide resolution CLIP (iCLIP) are methods to study protein-RNA interactions in untreated cells and tissues. Here, we analyzed six published and two novel data...
Analysis of CLIP and iCLIP methods for nucleotide-resolution studies of protein-RNA interactions
Yoichiro Sugimoto, Julian König, Shobbir Hussain, Blaž Zupan, Tomaž Curk, Michaela Frye, Jernej Ule
Jan 01, 0001
UV cross-linking and immunoprecipitation (CLIP) and individual-nucleotide resolution CLIP (iCLIP) are methods to study protein-RNA interactions in untreated cells and tissues. Here, we analyzed six published and two novel data...
Maternal and early life nutrition and physical activity
A Prioreschi, S Wrottesley, CE Draper, SA Tomaz, CJ Cook, ED Watson, MNM Van Poppel, R Said-Mohamed, SA Norris, EV Lambert, LK Micklesfield
Jan 21, 2020
Early life is important for later health outcomes, yet there are few studies which adequately address all of the potential early life insults that may affect later life health and growth trajectories. This is particularly...
Africa Public Health Developing Countries Infancy Pregnancy Adult Exercise Female Humans Infant Infant Nutritional Physiological Phenomena Infant Newborn Male Malnutrition Maternal Nutritional Physiological Phenomena Motor Activity Nutritional Status Pregnancy South Africa
Published by: Glob Health Action
Method to Synchronize Cell Cycle of Human Pluripotent Stem Cells without Affecting Their Fundamental Characteristics.
Loukia Yiangou, Rodrigo A Grandy, Carola M Morell, Rute A Tomaz, Anna Osnato, Juned Kadiwala, Daniele Muraro, Jose Garcia-Bernardo, Shota Nakanoh, William G Bernard, Daniel Ortmann, Davis J McCarthy, Ingrid Simonic, Sanjay Sinha, Ludovic Vallier
Jan 07, 2019
Liquid-crystalline ordering of antimicrobial peptide-DNA complexes controls TLR9 activation.
Nathan W Schmidt, Fan Jin, Roberto Lande, Tine Curk, Wujing Xian, Calvin Lee, Loredana Frasca, Daan Frenkel, Jure Dobnikar, Michel Gilliet, Gerard CL Wong
Jul 13, 2015
The RNA-binding protein HuR is essential for the B cell antibody response.
Manuel D Diaz-Muñoz, Sarah E Bell, Kirsten Fairfax, Elisa Monzon-Casanova, Adam F Cunningham, Mar Gonzalez-Porta, Simon R Andrews, Victoria I Bunik, Kathi Zarnack, Tomaž Curk, Ward A Heggermont, Stephane Heymans, Gary E Gibson, Dimitris L Kontoyiannis, Jernej Ule, Martin Turner
Jun 24, 2019
Post-transcriptional regulation of mRNA by the RNA-binding protein HuR (encoded by Elavl1) is required in B cells for the germinal center reaction and for the production of class-switched antibodies in response to...
Acyltransferases Alternative Splicing Animals Antigens B-Lymphocytes Cell Death Cell Differentiation Cell Proliferation ELAV Proteins Erythrocytes Germinal Center Immunity Humoral Immunization Immunoglobulin Class Switching Immunoglobulins Lipopolysaccharides Mice Mice Inbred C57BL Mice Knockout Mitochondria RNA Messenger Reactive Oxygen Species Sheep
Dynamic CpG methylation delineates subregions within super-enhancers selectively decommissioned at the exit from naive pluripotency.
Emma Bell, Edward W Curry, Wout Megchelenbrink, Luc Jouneau, Vincent Brochard, Rute A Tomaz, King Hang T Mau, Yaser Atlasi, Roshni A de Souza, Hendrik Marks, Hendrik G Stunnenberg, Alice Jouneau, Véronique Azuara
May 04, 2020
Clusters of enhancers, referred as to super-enhancers (SEs), control the expression of cell identity genes. The organisation of these clusters, and how they are remodelled upon developmental transitions remain poorly understood....
Generation of functional hepatocytes by forward programming with nuclear receptors.
Rute A Tomaz, Ekaterini D Zacharis, Fabian Bachinger, Annabelle Wurmser, Daniel Yamamoto, Sandra Petrus-Reurer, Carola M Morell, Dominika Dziedzicka, Brandon T Wesley, Imbisaat Geti, Charis-Patricia Segeritz, Miguel C de Brito, Mariya Chhatriwala, Daniel Ortmann, Kourosh Saeb-Parsy, Ludovic Vallier
Sep 12, 2022
Generation of functional hepatocytes by forward programming with nuclear receptors.
Rute A Tomaz, Ekaterini D Zacharis, Fabian Bachinger, Annabelle Wurmser, Daniel Yamamoto, Sandra Petrus-Reurer, Carola M Morell, Dominika Dziedzicka, Brandon T Wesley, Imbisaat Geti, Charis-Patricia Segeritz, Miguel C de Brito, Mariya Chhatriwala, Daniel Ortmann, Kourosh Saeb-Parsy, Ludovic Vallier
Aug 12, 2022
Computational design of probes to detect bacterial genomes by multivalent binding.
Tine Curk, Chris A Brackley, James D Farrell, Zhongyang Xing, Darshana Joshi, Susana Direito, Urban Bren, Stefano Angioletti-Uberti, Jure Dobnikar, Erika Eiser, Daan Frenkel, Rosalind J Allen
Oct 06, 2020
Rapid methods for diagnosis of bacterial infections are urgently needed to reduce inappropriate use of antibiotics, which contributes to antimicrobial resistance. In many rapid diagnostic methods, DNA oligonucleotide probes...
DNA-based detection computer simulations multivalent binding polymer physics superselectivity Computational Biology Computer Simulation Computer-Aided Design DNA Probes DNA Bacterial Genome Bacterial Oligonucleotide Array Sequence Analysis Oligonucleotide Probes Sensitivity and Specificity Sequence Analysis DNA
A systems view of spliceosomal assembly and branchpoints with iCLIP.
Michael Briese, Nejc Haberman, Christopher R Sibley, Rupert Faraway, Andrea S Elser, Anob M Chakrabarti, Zhen Wang, Julian König, David Perera, Vihandha O Wickramasinghe, Ashok R Venkitaraman, Nicholas M Luscombe, Luciano Saieva, Livio Pellizzoni, Christopher WJ Smith, Tomaž Curk, Jernej Ule
Apr 22, 2020
Studies of spliceosomal interactions are challenging due to their dynamic nature. Here we used spliceosome iCLIP, which immunoprecipitates SmB along with small nuclear ribonucleoprotein particles and auxiliary RNA binding...
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