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Results: 33
CyanoGate
Ravendran Vasudevan, Grant AR Gale, Alejandra A Schiavon, Anton Puzorjov, John Malin, Michael D Gillespie, Konstantinos Vavitsas, Valentin Zulkower, Baojun Wang, Christopher J Howe, David J Lea-Smith, Alistair J McCormick
Mar 14, 2019
Recent advances in synthetic biology research have been underpinned by an exponential increase in available genomic information and a proliferation of advanced DNA assembly tools. The adoption of plasmid vector assembly...
Analysis of the native structure, stability and aggregation of biotinylated human lysozyme.
Minkoo Ahn, Erwin De Genst, Gabriele S Kaminski Schierle, Miklos Erdelyi, Clemens F Kaminski, Christopher M Dobson, Janet R Kumita
Feb 12, 2020
Fibril formation by mutational variants of human lysozyme is associated with a fatal form of hereditary non-neuropathic systemic amyloidosis. Defining the mechanistic details of lysozyme aggregation is of crucial importance for...
Bioengineering horizon scan 2020.
Luke Kemp, Laura Adam, Christian R Boehm, Rainer Breitling, Rocco Casagrande, Malcolm Dando, Appolinaire Djikeng, Nicholas G Evans, Richard Hammond, Kelly Hills, Lauren A Holt, Todd Kuiken, Alemka Markotić, Piers Millett, Johnathan A Napier, Cassidy Nelson, Seán S ÓhÉigeartaigh, Anne Osbourn, Megan Palmer, Nicola J Patron, Edward Perello, Wibool Piyawattanametha, Vanessa Restrepo-Schild, Clarissa Rios-Rojas, Catherine Rhodes, Anna Roessing, Deborah Scott, Philip Shapira, Christopher Simuntala, Robert DJ Smith, Lalitha S Sundaram, Eriko Takano, Gwyn Uttmark, Bonnie Wintle, Nadia B Zahra, William J Sutherland
Aug 07, 2020
Horizon scanning is intended to identify the opportunities and threats associated with technological, regulatory and social change. In 2017 some of the present authors conducted a horizon scan for bioengineering (Wintle et al....
Toward a complete Drosophila deficiency kit.
Targeted chromosome engineering has facilitated the development of new deletions that now cover virtually the entire genome of Drosophila, providing greater precision and resolution.
Characterisation of Drosophila Ubx CPTI000601 and hth CPTI000378 protein trap lines.
Intestines Intestinal Mucosa Embryo Nonmammalian Animals Drosophila melanogaster Bacterial Proteins Homeodomain Proteins Drosophila Proteins Luminescent Proteins Recombinant Fusion Proteins Transcription Factors Genetic Engineering Gene Expression Regulation Developmental Gene Deletion Founder Effect Genotype Homozygote Phenotype Genes Reporter Imaginal Discs
Published by: TheScientificWorldJournal
Modulating Vaccinia Virus Immunomodulators to Improve Immunological Memory.
The increasing frequency of monkeypox virus infections, new outbreaks of other zoonotic orthopoxviruses and concern about the re-emergence of smallpox have prompted research into developing antiviral drugs and better vaccines...
Genetic Engineering Immune Evasion immunological memory orthopoxviruses smallpox vaccine vaccinia virus Animals Communicable Diseases Emerging Cytokines Genetic Engineering Humans Immunity Immunogenicity Vaccine Immunologic Memory immunomodulation Inflammation Mediators smallpox smallpox vaccine vaccinia virus Viral Vaccines Zoonoses
Characterisation of Drosophila Ubx CPTI000601 and hth CPTI000378 protein trap lines.
Animals Bacterial Proteins Drosophila Proteins Drosophila melanogaster Embryo Nonmammalian Founder Effect Gene Deletion Gene Expression Regulation Developmental Genes Reporter Genetic Engineering Genotype Homeodomain Proteins Homozygote Imaginal Discs Intestinal Mucosa Intestines Luminescent Proteins Phenotype Recombinant Fusion Proteins Transcription Factors
The design and in vivo evaluation of engineered I-OnuI-based enzymes for HEG gene drive.
Yuk-Sang Chan, Ryo Takeuchi, Jordan Jarjour, David S Huen, Barry L Stoddard, Steven Russell
Mar 29, 2018
The homing endonuclease gene (HEG) drive system, a promising genetic approach for controlling arthropod populations, utilises engineered nucleases to spread deleterious mutations that inactivate individual genes throughout a...
Standards for plant synthetic biology
Nicola J Patron, Diego Orzaez, Sylvestre Marillonnet, Heribert Warzecha, Colette Matthewman, Mark Youles, Oleg Raitskin, Aymeric Leveau, Gemma Farré, Christian Rogers, Alison Smith, Julian Hibberd, Alex AR Webb, James Locke, Sebastian Schornack, Jim Ajioka, David C Baulcombe, Cyril Zipfel, Sophien Kamoun, Jonathan DG Jones, Hannah Kuhn, Silke Robatzek, H Peter van Esse, Dale Sanders, Giles Oldroyd, Cathie Martin, Rob Field, Sarah O'Connor, Samantha Fox, Brande Wulff, Ben Miller, Andy Breakspear, Guru Radhakrishnan, Pierre-Marc Delaux, Dominique Loqué, Antonio Granell, Alain Tissier, Patrick Shih, Thomas P Brutnell, W Paul Quick, Heiko Rischer, Paul D Fraser, Asaph Aharoni, Christine Raines, Paul F South, Jean-Michel Ané, Björn R Hamberger, Jane Langdale, Jens Stougaard, Harro Bouwmeester, Michael Udvardi, James AH Murray, Vardis Ntoukakis, Patrick Schäfer, Katherine Denby, Keith J Edwards, Anne Osbourn, Jim Haseloff
Jun 13, 2019
DNA assembly GoldenGate Type IIS restriction endonucleases Cloning genetic syntax synthetic biology Botany Cloning Molecular DNA Deoxyribonucleases Type II Site-Specific Eukaryota Genetic Engineering Plants Plants Genetically Modified Plasmids Reference Standards synthetic biology Transcription Genetic
Programmed hierarchical patterning of bacterial populations.
Modern genetic tools allow the dissection and emulation of fundamental mechanisms shaping morphogenesis in multicellular organisms. Several synthetic genetic circuits for control of multicellular patterning have been reported to...
Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats.
Toshihiro Kobayashi, Teppei Goto, Mami Oikawa, Makoto Sanbo, Fumika Yoshida, Reiko Terada, Naoko Niizeki, Naoyo Kajitani, Kanako Kazuki, Yasuhiro Kazuki, Shinichi Hochi, Hiromitsu Nakauchi, M Azim Surani, Masumi Hirabayashi
Apr 16, 2021
Murine animal models from genetically modified pluripotent stem cells (PSCs) are essential for functional genomics and biomedical research, which require germline transmission for the establishment of colonies. However, the...
BIOSAFETY. Safeguarding gene drive experiments in the laboratory.
Omar S Akbari, Hugo J Bellen, Ethan Bier, Simon L Bullock, Austin Burt, George M Church, Kevin R Cook, Peter Duchek, Owain R Edwards, Kevin M Esvelt, Valentino M Gantz, Kent G Golic, Scott J Gratz, Melissa M Harrison, Keith R Hayes, Anthony A James, Thomas C Kaufman, Juergen Knoblich, Harmit S Malik, Kathy A Matthews, Kate M O'Connor-Giles, Annette L Parks, Norbert Perrimon, Fillip Port, Steven Russell, Ryu Ueda, Jill Wildonger
Sep 16, 2015
High molecular weight DNA assembly in vivo for synthetic biology applications.
Synthetic genome recoding
James Kuo, Finn Stirling, Yu Heng Lau, Yekaterina Shulgina, Jeffrey C Way, Pamela A Silver
Nov 02, 2020
Full genome recoding, or rewriting codon meaning, through chemical synthesis of entire bacterial chromosomes has become feasible in the past several years. Recoding an organism can impart new properties including non-natural...
Engineering a Model Cell for Rational Tuning of GPCR Signaling.
William M Shaw, Hitoshi Yamauchi, Jack Mead, Glen-Oliver F Gowers, David J Bell, David Öling, Niklas Larsson, Mark Wigglesworth, Graham Ladds, Tom Ellis
Mar 08, 2019
G protein-coupled receptor Saccharomyces cerevisiae Biosensor Cell Signaling cell-to-cell communication genome engineering synthetic biology Gene Expression Genetic Engineering Humans Pheromones Receptors G-Protein-Coupled Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Transcription Factors
Evolutionary programming as a platform for in silico metabolic engineering.
BACKGROUND: Through genetic engineering it is possible to introduce targeted genetic changes and hereby engineer the metabolism of microbial cells with the objective to obtain desirable phenotypes. However, owing to the...
Algorithms Benzaldehydes Computational Biology Computer Simulation Escherichia coli evolution Molecular Gene Expression Regulation Genetic Engineering Genome Fungal Glycerol Models Biological Models Genetic Phenotype Protein Engineering Protein Interaction Mapping Saccharomyces cerevisiae Software Succinic Acid
Precise optical control of gene expression in C elegans using improved genetic code expansion and Cre recombinase.
Lloyd Davis, Inja Radman, Angeliki Goutou, Ailish Tynan, Kieran Baxter, Zhiyan Xi, Jack M O'Shea, Jason W Chin, Sebastian Greiss
Oct 26, 2021
Synthetic strategies for optically controlling gene expression may enable the precise spatiotemporal control of genes in any combination of cells that cannot be targeted with specific promoters. We develop an improved genetic...
A new generation of T7 RNA polymerase-independent inducible expression plasmids for Trypanosoma brucei.
Expression of transgenes is central to forward and reverse genetic analysis in Trypanosoma brucei. The inducible expression of transgenes in trypanosomes is based on the tetracycline repressor binding to a tetracycline operator...
Reverse genetic strategies in Caenorhabditis elegans
Caenorhabditis elegans has a complete annotated genome sequence that is augmented by increasing quantities of data from high-throughput postgenomic analyses. This has led to an increasing need to identify the biological...
Immunological considerations and challenges for regenerative cellular therapies.
Sandra Petrus-Reurer, Marco Romano, Sarah Howlett, Joanne Louise Jones, Giovanna Lombardi, Kourosh Saeb-Parsy
Jun 16, 2021
The central goal of regenerative medicine is to replace damaged or diseased tissue with cells that integrate and function optimally. The capacity of pluripotent stem cells to produce unlimited numbers of differentiated cells is...
Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation.
Laura Broutier, Amanda Andersson-Rolf, Christopher J Hindley, Sylvia F Boj, Hans Clevers, Bon-Kyoung Koo, Meritxell Huch
Jun 28, 2016
Doris Gangl, Julie AZ Zedler, Priscilla D Rajakumar, Erick M Ramos Martinez, Anthony Riseley, Artur Włodarczyk, Saul Purton, Yumiko Sakuragi, Christopher J Howe, Poul Erik Jensen, Colin Robinson
Sep 07, 2015
Correlation between the binding affinity and the conformational entropy of nanobody SARS-CoV-2 spike protein complexes.
Halina Mikolajek, Miriam Weckener, Z Faidon Brotzakis, Jiandong Huo, Evmorfia V Dalietou, Audrey Le Bas, Pietro Sormanni, Peter J Harrison, Philip N Ward, Steven Truong, Lucile Moynie, Daniel K Clare, Maud Dumoux, Joshua Dormon, Chelsea Norman, Naveed Hussain, Vinod Vogirala, Raymond J Owens, Michele Vendruscolo, James H Naismith
Aug 20, 2022
Camelid single-domain antibodies, also known as nanobodies, can be readily isolated from naïve libraries for specific targets but often bind too weakly to their targets to be immediately useful. Laboratory-based genetic...
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