Search
Results: 124
Targeted DamID reveals differential binding of mammalian pluripotency factors.
Seth W Cheetham, Wolfram H Gruhn, Jelle van den Ameele, Robert Krautz, Tony D Southall, Toshihiro Kobayashi, M Azim Surani, Andrea H Brand
Oct 03, 2018
The precise control of gene expression by transcription factor networks is crucial to organismal development. The predominant approach for mapping transcription factor-chromatin interactions has been chromatin...
Inhibition of the RNA polymerase III-mediated dsDNA-sensing pathway of innate immunity by vaccinia virus protein E3.
The vaccinia virus E3 protein is an important intracellular modulator of innate immunity that can be split into distinct halves. The C terminus contains a well defined dsRNA-binding domain, whereas the N terminus contains a...
Physical and neurobehavioral determinants of reproductive onset and success.
FR Day, H Helgason, DI Chasman, LM Rose, P-R Loh, RA Scott, A Helgason, A Kong, G Masson, OT Magnusson, D Gudbjartsson, U Thorsteinsdottir, JE Buring, PM Ridker, P Sulem, K Stefansson, KK Ong, JRB Perry
Mar 15, 2016
Localized Translation of gurken/TGF-α mRNA during Axis Specification Is Controlled by Access to Orb/CPEB on Processing Bodies.
CARM1 and Paraspeckles Regulate Pre-implantation Mouse Embryo Development.
Anna Hupalowska, Agnieszka Jedrusik, Meng Zhu, Mark T Bedford, David M Glover, Magdalena Zernicka-Goetz
Dec 21, 2018
Nuclear architecture has never been carefully examined during early mammalian development at the stages leading to establishment of the embryonic and extra-embryonic lineages. Heterogeneous activity of the methyltransferase...
CARM1 Cell fate development Embryo Heterogeneity nuclear bodies p54/nrb paraspeckles phase-separation pre-implantation Animals Blastocyst Cell Cycle Checkpoints Cell Differentiation Cell Lineage Embryonic Development Mice Nuclear Matrix-Associated Proteins Protein-Arginine N-Methyltransferases RNA Long Noncoding RNA-Binding Proteins
RNA-DamID reveals cell-type-specific binding of roX RNAs at chromatin-entry sites.
Thousands of long noncoding RNAs (lncRNAs) have been identified in eukaryotic genomes, many of which are expressed in spatially and temporally restricted patterns. Nonetheless, the roles of the majority of these transcripts are...
Genetic variants in PPARGC1B and CNTN4 are associated with thromboxane A2 formation and with cardiovascular event free survival in the Anglo-Scandinavian Cardiac Outcomes Trial (ASCOT).
Nina S McCarthy, Ciara Vangjeli, Praveen Surendran, Achim Treumann, Cathy Rooney, Emily Ho, Peter Sever, Simon Thom, Alun D Hughes, Patricia B Munroe, Philip Howard, Toby Johnson, Mark Caulfield, Denis C Shields, Eoin O'Brien, Desmond J Fitzgerald, Alice V Stanton
Mar 16, 2018
BACKGROUND AND AIMS: Elevated urinary 11-dehydro thromboxane B2 (TxB2), a measure of thromboxane A2 formation in vivo, predicts future atherothrombotic events. To further understand this relationship, the genetic determinants of...
Genome wide association study Thrombosis Thromboxane Aged Aspirin Cardiovascular Agents Cardiovascular Diseases Carrier Proteins Contactins Europe Female Gene Frequency Genetic Markers Genetic Predisposition to Disease Genome-Wide Association Study Haplotypes Humans Incidence Male Middle Aged Multicenter Studies as Topic Phenotype Polymorphism Single Nucleotide Primary Prevention Progression-Free Survival RNA-Binding Proteins Randomized Controlled Trials as Topic Risk Factors Thromboxane A2 Thromboxane B2 Time Factors White People
Developmental characterization of the microRNA-specific C. elegans Argonautes alg-1 and alg-2.
Alejandro Vasquez-Rifo, Guillaume Jannot, Javier Armisen, Michel Labouesse, Syed Irfan Ahmad Bukhari, Evelyne L Rondeau, Eric A Miska, Martin J Simard
Sep 27, 2018
The genes alg-1 and alg-2 (referred to as "alg-1/2") encode the Argonaute proteins affiliated to the microRNA (miRNA) pathway in C. elegans. Bound to miRNAs they form the effector complex that effects post-transcriptional gene...
The RNA-binding proteins Zfp36l1 and Zfp36l2 act redundantly in myogenesis.
Hema Bye-A-Jee, Dhamayanthi Pugazhendhi, Samuel Woodhouse, Patrick Brien, Rachel Watson, Martin Turner, Jennifer Pell
Jun 24, 2019
BACKGROUND: Members of the ZFP36 family of RNA-binding proteins regulate gene expression post-transcriptionally by binding to AU-rich elements in the 3'UTR of mRNA and stimulating mRNA degradation. The proteins within this...
Injury RNA RNA-Binding Proteins Regeneration satellite cells Skeletal muscle stem cells Zfp36L1 Zfp36L2 Animals Butyrate Response Factor 1 Cell Differentiation Cells Cultured Female Male Mice Mice Inbred C57BL Muscle Development Muscle Skeletal Nuclear Proteins PAX7 Transcription Factor RNA Messenger RNA-Binding Proteins satellite cells Skeletal Muscle Tristetraprolin
Identification of the RNA polymerase I-RNA interactome.
David Piñeiro, Mark Stoneley, Manasa Ramakrishna, Jana Alexandrova, Veronica Dezi, Rebekha Juke-Jones, Kathryn S Lilley, Kelvin Cain, Anne E Willis
Mar 21, 2019
Ribosome biogenesis is a complex process orchestrated by a host of ribosome assembly factors. Although it is known that many of the proteins involved in this process have RNA binding activity, the full repertoire of proteins...
Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.
Max Koppers, Roberta Cagnetta, Toshiaki Shigeoka, Lucia Cs Wunderlich, Pedro Vallejo-Ramirez, Julie Qiaojin Lin, Sixian Zhao, Maximilian AH Jakobs, Asha Dwivedy, Michael S Minett, Anaïs Bellon, Clemens F Kaminski, William A Harris, John G Flanagan, Christine E Holt
Nov 25, 2019
Extrinsic cues trigger the local translation of specific mRNAs in growing axons via cell surface receptors. The coupling of ribosomes to receptors has been proposed as a mechanism linking signals to local translation but it is...
RNA-binding protein axon developmental biology guidance receptor human local protein synthesis mRNA neuroscience Retinal ganglion cell Xenopus Animals Axons Protein Biosynthesis Proteome RNA Messenger RNA-Binding Proteins Receptors Cell Surface Retinal Ganglion Cells Ribosomes Signal Transduction Xenopus laevis
The mTOR regulated RNA-binding protein LARP1 requires PABPC1 for guided mRNA interaction.
Ewan M Smith, Nour El Houda Benbahouche, Katherine Morris, Ania Wilczynska, Sarah Gillen, Tobias Schmidt, Hedda A Meijer, Rebekah Jukes-Jones, Kelvin Cain, Carolyn Jones, Mark Stoneley, Joseph A Waldron, Cameron Bell, Bruno D Fonseca, Sarah Blagden, Anne E Willis, Martin Bushell
Feb 26, 2021
The mammalian target of rapamycin (mTOR) is a critical regulator of cell growth, integrating multiple signalling cues and pathways. Key among the downstream activities of mTOR is the control of the protein synthesis machinery....
5' Untranslated Regions Autoantigens Gene Expression Regulation Genes Reporter HeLa Cells Humans Mechanistic Target of Rapamycin Complex 1 Mechanistic Target of Rapamycin Complex 2 Mutagenesis Site-Directed Mutation Missense Naphthyridines Point Mutation Poly(A)-Binding Protein I Polyribosomes Protein Biosynthesis RNA Interference RNA Messenger RNA-Binding Proteins Recombinant Fusion Proteins Ribonucleoproteins TOR Serine-Threonine Kinases
CRISPR-assisted detection of RNA-protein interactions in living cells.
Wenkai Yi, Jingyu Li, Xiaoxuan Zhu, Xi Wang, Ligang Fan, Wenju Sun, Linbu Liao, Jilin Zhang, Xiaoyu Li, Jing Ye, Fulin Chen, Jussi Taipale, Kui Ming Chan, Liang Zhang, Jian Yan
Jan 11, 2021
We have developed CRISPR-assisted RNA-protein interaction detection method (CARPID), which leverages CRISPR-CasRx-based RNA targeting and proximity labeling to identify binding proteins of specific long non-coding RNAs (lncRNAs)...
A Regulatory Axis between Epithelial Splicing Regulatory Proteins and Estrogen Receptor α Modulates the Alternative Transcriptome of Luminal Breast Cancer.
Jamal Elhasnaoui, Giulio Ferrero, Valentina Miano, Lorenzo Franchitti, Isabella Tarulli, Lucia Coscujuela Tarrero, Santina Cutrupi, Michele De Bortoli
Sep 02, 2022
Epithelial splicing regulatory proteins 1 and 2 (ESRP1/2) control the splicing pattern during epithelial to mesenchymal transition (EMT) in a physiological context and in cancer, including breast cancer (BC). Here, we report...
EMT splicing signature ESRP1 ESRP2 Rac1 Rac1b Alternative Splicing Breast Cancer Alternative Splicing Breast Neoplasms Cell Line Tumor Epithelial-Mesenchymal Transition Estrogen Receptor alpha Female Gene Expression Regulation Neoplastic Humans RNA-Binding Proteins Transcription Factors Transcriptome
Depletion of the RNA-binding protein RBP33 results in increased expression of silenced RNA polymerase II transcripts in Trypanosoma brucei.
Full-length NF-κB repressing factor contains an XRN2 binding domain.
Jana Alexandrova, David Piñeiro, Rebekah Jukes-Jones, Ryan Mordue, Mark Stoneley, Anne E Willis
Mar 06, 2020
NF-κB repressing factor (NKRF) was recently identified as an RNA binding protein that together with its associated proteins, the 5'-3' exonuclease XRN2 and the helicase DHX15, is required to process the precursor ribosomal RNA....
Malonylation of GAPDH is an inflammatory signal in macrophages.
Silvia Galván-Peña, Richard G Carroll, Carla Newman, Elizabeth C Hinchy, Eva Palsson-McDermott, Elektra K Robinson, Sergio Covarrubias, Alan Nadin, Andrew M James, Moritz Haneklaus, Susan Carpenter, Vincent P Kelly, Michael P Murphy, Louise K Modis, Luke A O'Neill
Feb 12, 2019
Macrophages undergo metabolic changes during activation that are coupled to functional responses. The gram negative bacterial product lipopolysaccharide (LPS) is especially potent at driving metabolic reprogramming, enhancing...
Animals Cytokines Glyceraldehyde-3-Phosphate Dehydrogenases HEK293 Cells Humans Inflammation Inflammation Mediators Lipopolysaccharides Lysine MACROPHAGES Malonyl Coenzyme A Mice Inbred C57BL Mutagenesis Polyribosomes RNA Messenger RNA Small Interfering RNA-Binding Proteins Tumor Necrosis Factor-alpha
The physiological and pathological biophysics of phase separation and gelation of RNA binding proteins in amyotrophic lateral sclerosis and fronto-temporal lobar degeneration.
Peter St George-Hyslop, Julie Qiaojin Lin, Akinori Miyashita, Emma C Phillips, Seema Qamar, Suzanne J Randle, GuoZhen Wang
Sep 05, 2018
Amyotrophic Lateral Sclerosis arginine methylation Cation-pi interactions frontotemporal dementia phase separation RNA binding proteins Amyotrophic Lateral Sclerosis biophysics Cytoplasmic Granules DNA-Binding Proteins frontotemporal dementia Frontotemporal Lobar Degeneration Humans Mutation Neurodegenerative Diseases Protein Domains Protein Processing Post-Translational RNA-Binding Protein FUS RNA-Binding Proteins Temporal Lobe
Viroplasms
Viroplasms are cytoplasmic, membraneless structures assembled in rotavirus (RV)-infected cells, which are intricately involved in viral replication. Two virus-encoded, non-structural proteins, NSP2 and NSP5, are the main drivers...
Crispr-Csy4 Genome Editing Nsp2 Nsp5 liquid-liquid phase separation Protein-Rna Condensates Replication Cycle Reverse Genetics Rotavirus Viroplasm Animals Capsid Proteins Cytoplasm Cytosol Humans Phosphorylation RNA-Binding Proteins Rotavirus Rotavirus Infections Viral Nonstructural Proteins Viral Replication Compartments Virus Assembly Virus Replication
Polypyrimidine tract-binding proteins are essential for B cell development
Elisa Monzón-Casanova, Louise S Matheson, Kristina Tabbada, Kathi Zarnack, Christopher WJ Smith, Martin Turner
Mar 06, 2020
Polypyrimidine tract-binding protein 1 (PTBP1) is a RNA-binding protein (RBP) expressed throughout B cell development. Deletion of Ptbp1 in mouse pro-B cells results in upregulation of PTBP2 and normal B cell development. We...
Drought Stress Causes Specific Changes to the Spliceosome and Stress Granule Components.
The spliceosome processes RNAs from a pre-RNA state to a mature mRNA thereby influencing RNA availability for translation, localization, and turnover. It consists of complex structures containing RNA-binding proteins (RBPs)...
Drought stress Systems Analysis RNA-Binding Proteins Spliceosome Stress granules Mrna Interactome Capture
Published by: Frontiers in molecular biosciences
|
|< |
< |
1 |