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Results: 55
Direct evidence of milk consumption from ancient human dental calculus.
C Warinner, J Hendy, C Speller, E Cappellini, R Fischer, C Trachsel, J Arneborg, N Lynnerup, OE Craig, DM Swallow, A Fotakis, RJ Christensen, JV Olsen, A Liebert, N Montalva, S Fiddyment, S Charlton, M Mackie, A Canci, A Bouwman, F Rühli, MTP Gilbert, MJ Collins
Nov 22, 2018
Milk is a major food of global economic importance, and its consumption is regarded as a classic example of gene-culture evolution. Humans have exploited animal milk as a food resource for at least 8500 years, but the origins...
Proteomic plasma membrane profiling reveals an essential role for gp96 in the cell surface expression of LDLR family members, including the LDL receptor and LRP6.
Michael P Weekes, Robin Antrobus, Suzanne Talbot, Simon Hör, Nikol Simecek, Duncan L Smith, Stuart Bloor, Felix Randow, Paul J Lehner
May 29, 2019
The endoplasmic reticulum chaperone gp96 is required for the cell surface expression of a narrow range of proteins, including toll-like receptors (TLRs) and integrins. To identify a more comprehensive repertoire of proteins...
Animals Antigens CD Cell Line Cell Membrane Chromatography High Pressure Liquid Down-Regulation Integrins Low Density Lipoprotein Receptor-Related Protein-6 Membrane Glycoproteins Membrane Proteins Mice Peptide Fragments Protein Interaction Mapping Proteomics Receptors LDL Tandem Mass Spectrometry Toll-Like Receptors
A computational platform for high-throughput analysis of RNA sequences and modifications by mass spectrometry.
Samuel Wein, Byron Andrews, Timo Sachsenberg, Helena Santos-Rosa, Oliver Kohlbacher, Tony Kouzarides, Benjamin A Garcia, Hendrik Weisser
Apr 14, 2020
The field of epitranscriptomics continues to reveal how post-transcriptional modification of RNA affects a wide variety of biological phenomena. A pivotal challenge in this area is the identification of modified RNA residues...
Formation and abundance of 5-hydroxymethylcytosine in RNA.
Sabrina M Huber, Pieter van Delft, Lee Mendil, Martin Bachman, Katherine Smollett, Finn Werner, Eric A Miska, Shankar Balasubramanian
May 12, 2015
Functionalized graphene oxide tablets for sample preparation of drugs in biological fluids
Zeynab Zohdijamil, Mahdi Hashemi, Abbi Abdel-Rehim, Karthik Laxman, Abdusalam Uheida, Joydeep Dutta, Mohamed Abdel-Rehim
Jun 04, 2021
In this work, graphene oxide-based tablets (GO-Tabs) were prepared by applying a thin layer of functionalized GO on a polyethylene substrate. The GO was functionalized with amine groups (-NH2 ) by poly(ethylene...
Serendipitous Identification of a Covalent Activator of Liver Pyruvate Kinase.
Umberto Maria Battisti, Chunxia Gao, Oscar Nilsson, Fady Akladios, Aleksei Lulla, Agnieszka Bogucka, Amalyn Nain-Perez, Liliana Håversen, Woonghee Kim, Jan Boren, Marko Hyvönen, Mathias Uhlen, Adil Mardinoglu, Morten Grøtli
Nov 09, 2022
Cell-selective labeling using amino acid precursors for proteomic studies of multicellular environments.
Nicholas P Gauthier, Boumediene Soufi, William E Walkowicz, Virginia A Pedicord, Konstantinos J Mavrakis, Boris Macek, David Y Gin, Chris Sander, Martin L Miller
Jan 24, 2018
We report a technique to selectively and continuously label the proteomes of individual cell types in coculture, named cell type-specific labeling using amino acid precursors (CTAP). Through transgenic expression of exogenous...
Organoid Sample Preparation and Extraction for LC-MS Peptidomics.
Emily L Miedzybrodzka, Rachel E Foreman, Sam G Galvin, Pierre Larraufie, Amy L George, Deborah A Goldspink, Frank Reimann, Fiona M Gribble, Richard G Kay
Mar 18, 2021
This protocol describes the peptidomic analysis of organoid lysates, FACS-purified cell populations, and 2D culture secretions by liquid chromatography mass spectrometry (LC-MS). Currently, most peptides are quantified by ELISA...
Tailored Mobility in a Zeolite Imidazolate Framework (ZIF) Antibody Conjugate*.
Ander Chapartegui-Arias, Anna Raysyan, Ana M Belenguer, Carsten Jaeger, Teodor Tchipilov, Carsten Prinz, Carlos Abad, Sebastian Beyer, Rudolf J Schneider, Franziska Emmerling
May 21, 2021
Zeolitic imidazolate framework (ZIF) hybrid fluorescent nanoparticles and ZIF antibody conjugates have been synthesized, characterized, and employed in lateral-flow immunoassay (LFIA). The bright fluorescence of the conjugates...
Impact of Irradiation on the Pharmacokinetics and Biotransformation of Tamoxifen.
Yung-Yi Cheng, Teresa Zheng, Michael W Chang, Jeffrey W Dalley, Yu-Jen Chen, Tung-Hu Tsai, Chen-Hsi Hsieh
Mar 03, 2022
BACKGROUND: The optimal procedure for combining radiotherapy (RT) with tamoxifen treatment is controversial as RT may alter the pharmacokinetics and biotransformation of tamoxifen. The present study investigated this potential...
Enhanced Methylation Analysis by Recovery of Unsequenceable Fragments.
Gordon R McInroy, Dario Beraldi, Eun-Ang Raiber, Katarzyna Modrzynska, Pieter van Delft, Oliver Billker, Shankar Balasubramanian
Apr 07, 2016
Efficient and irreversible antibody-cysteine bioconjugation using carbonylacrylic reagents.
Barbara Bernardim, Maria J Matos, Xhenti Ferhati, Ismael Compañón, Ana Guerreiro, Padma Akkapeddi, Antonio CB Burtoloso, Gonzalo Jiménez-Osés, Francisco Corzana, Gonçalo JL Bernardes
May 08, 2019
Measurement of the total angiotensinogen and its reduced and oxidised forms in human plasma using targeted LC-MS/MS.
Lina A Dahabiyeh, David Tooth, Robin W Carrell, Randy J Read, Yahui Yan, Fiona Broughton Pipkin, David A Barrett
Dec 11, 2018
Angiotensinogen (AGT) is a critical protein in the renin-angiotensin-aldosterone system and may have an important role in the pathogenesis of pre-eclampsia. The disulphide linkage between cysteines 18 and 138 has a key role in...
In vivo analysis of proteomes and interactomes using Parallel Affinity Capture (iPAC) coupled to mass spectrometry.
Johanna S Rees, Nick Lowe, Irina M Armean, John Roote, Glynnis Johnson, Emma Drummond, Helen Spriggs, Edward Ryder, Steven Russell, Daniel St Johnston, Kathryn S Lilley
Jan 18, 2016
A sensitive mass spectrometric assay for mitochondrial CoQ pool redox state in vivo.
Nils Burger, Angela Logan, Tracy A Prime, Amin Mottahedin, Stuart T Caldwell, Thomas Krieg, Richard C Hartley, Andrew M James, Michael P Murphy
Oct 07, 2021
Coenzyme Q (CoQ) is an essential cofactor, primarily found in the mitochondrial inner membrane where it functions as an electron carrier in the respiratory chain, and as a lipophilic antioxidant. The redox state of the CoQ pool...
The Dissociation of Gefitinib Trough Concentration and Clinical Outcome in NSCLC Patients with EGFR Sensitive Mutations.
Shuang Xin, Yuanyuan Zhao, Xueding Wang, Yan Huang, Jing Zhang, Ying Guo, Jiali Li, Hongliang Li, Yuxiang Ma, Lingyan Chen, Zhihuang Hu, Min Huang, Li Zhang
Nov 26, 2019
Gefitinib is an essential drug for NSCLC patients harboring EGFR sensitive mutations. The approved dose 250 mg/day is based on limited clinical trials, this research aims to explore the relationship between drug exposure and...
Leptin-Mediated Changes in the Human Metabolome.
Katherine Lawler, Isabel Huang-Doran, Takuhiro Sonoyama, Tinh-Hai Collet, Julia M Keogh, Elana Henning, Stephen O'Rahilly, Leonardo Bottolo, I Sadaf Farooqi
May 15, 2020
CONTEXT: While severe obesity due to congenital leptin deficiency is rare, studies in patients before and after treatment with leptin can provide unique insights into the role that leptin plays in metabolic and endocrine...
bile acids Leptin Lipids Metabolomics obesity Adolescent Child Child Preschool Chromatography Liquid Energy Intake Energy Metabolism Female Hormone Replacement Therapy Humans Leptin Lipolysis Loss of Function Mutation Male Metabolome Metabolomics obesity Recombinant Proteins Severity of Illness Index Tandem Mass Spectrometry Treatment Outcome
Quantitative mass spectrometry for human melanocortin peptides in vitro and in vivo suggests prominent roles for β-MSH and desacetyl α-MSH in energy homeostasis.
Peter Kirwan, Richard G Kay, Bas Brouwers, Vicente Herranz-Pérez, Magdalena Jura, Pierre Larraufie, Julie Jerber, Jason Pembroke, Theresa Bartels, Anne White, Fiona M Gribble, Frank Reimann, I Sadaf Farooqi, Stephen O'Rahilly, Florian T Merkle
Nov 13, 2018
OBJECTIVE: The lack of pro-opiomelanocortin (POMC)-derived melanocortin peptides results in hypoadrenalism and severe obesity in both humans and rodents that is treatable with synthetic melanocortins. However, there are...
Human pluripotent stem cell Leptin MSH Neuropeptide obesity POMC Chromatography Liquid Female Homeostasis Humans Hypothalamus Leptin Male Mass Spectrometry Melanocortins Neurons Neuropeptides Pluripotent Stem Cells Pro-Opiomelanocortin Receptors Melanocortin Tandem Mass Spectrometry alpha-MSH beta-MSH
Deciphering the genetic basis for polyketide variation among mycobacteria producing mycolactones.
Sacha J Pidot, Hui Hong, Torsten Seemann, Jessica L Porter, Marcus J Yip, Artem Men, Matthew Johnson, Peter Wilson, John K Davies, Peter F Leadlay, Timothy P Stinear
Jun 14, 2011
High-Resolution Mass Spectrometry Based Proteomic Analysis of the Response to Vancomycin-Induced Cell Wall Stress in Streptomyces coelicolor A3(2).
β-Hydroxybutyrate Accumulates in the Rat Heart during Low-Flow Ischaemia with Implications for Functional Recovery
Ross Lindsay, Sophie Dieckmann, Dominika Krzyzanska, Dominic Manetta-Jones, James West, Cecilia Castro, Julian Griffin, Andrew Murray
Aug 24, 2021
Heart ischaemia Langendorff Biochemistry cardiomyocyte Cell Biology chemical biology rat 3-Hydroxybutyric Acid Animals Chromatography Liquid Citric Acid Cycle Heart Hydroxymethylglutaryl-CoA Synthase Ischemia ketone bodies Male Mitochondria Myocardial Ischemia Myocytes Cardiac Oxidation-Reduction Rats Rats Wistar Tandem Mass Spectrometry
Proteomic systems evaluation of the molecular validity of preclinical psychosis models compared to schizophrenia brain pathology.
David A Cox, Michael G Gottschalk, Hendrik Wesseling, Agnes Ernst, Jason D Cooper, Sabine Bahn
Jul 07, 2016
chronic phencyclidine (PCP) rat model Molecular validity Proteomics Psychosis models Schizophrenia Systems Biology Animals Chromatography Liquid Disease Models Animal Humans Ketamine Machine Learning Male Mice Transgenic Phencyclidine Prefrontal Cortex Protein Interaction Maps Proteomics Psychotic Disorders Rats Receptors N-Methyl-D-Aspartate Schizophrenia Tandem Mass Spectrometry
Mass spectrometric characterisation of the circulating peptidome following oral glucose ingestion in control and gastrectomised patients.
Richard G Kay, Rachel E Foreman, Geoff P Roberts, Richard Hardwick, Frank Reimann, Fiona M Gribble
Jun 04, 2020
Multivariate proteomic profiling identifies novel accessory proteins of coated vesicles.
Georg HH Borner, Robin Antrobus, Jennifer Hirst, Gary S Bhumbra, Patrycja Kozik, Lauren P Jackson, Daniela A Sahlender, Margaret S Robinson
Jan 24, 2018
Despite recent advances in mass spectrometry, proteomic characterization of transport vesicles remains challenging. Here, we describe a multivariate proteomics approach to analyzing clathrin-coated vesicles (CCVs) from HeLa...
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